Reviewed, UniProtKB/Swiss-Prot Q5PBX6 (PYRG_ANAMM)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	AM018

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	CTP synthase HAMAP MF_01227		PRO_0000266054
Regions
	Domain	297 – 539	243	Glutamine amidotransferase type-1
	Region	1 – 259	259	Aminator domain HAMAP MF_01227
Sites
	Active site	383	1	Nucleophile By similarity
	Active site	512	1	By similarity
	Active site	514	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PBX6-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: D64F80BDE27F74D2	FASTA	560	61,146
	10 20 30 40 50 60 MSIRDGCSAR FIFVTGGVVS SLGKGLAAAS IGALLQARGF RVRLRKLDPY LNVDPGTMSP 70 80 90 100 110 120 AQHGEVFVTD DGGETDLDLG NYERFTGVNT TKEDNITAGR IYQQLLAKER RGDYLGHTVQ 130 140 150 160 170 180 VIPHVTDLII SFILSNDDGA DFIICEIGGT VGDIESQPFL ESIRQVSYRL SKNFTIFVHL 190 200 210 220 230 240 TLVPCVGSAG ELKTKPTQHS VKELSSLGIQ PDIILYRSAE PLPQYQSAKI ANFCNVSADN 250 260 270 280 290 300 VIPALDVESM YKLPVMYHAH KLDTQILSHF GMGAPEPDLT KWANVLTMVN NARNVVTIAI 310 320 330 340 350 360 IGKYTKFLDA YTSLTEALDH AGMHSGIKIQ VKWVDSRLPV RESDLHDVDG VLIPGGFGDD 370 380 390 400 410 420 GVDGKVLAIG YARANGIPML GICMGMQLAA IEFALNVAKL EDANSTEFNQ ACKNPIVVEL 430 440 450 460 470 480 PWLQKGEGEY LLGGSMRLGS CTYRLSADSR VASVYGSTVI NERCRHRYCI NPQYKNVLEE 490 500 510 520 530 540 HGLSFTGMSD SHGLVEVLEL QSHPWFIGVQ FHPEFKSSPF APHPLFTSFV QNVLQIKQRG 550 560 FMHKSVSAAA ILVPGSSVVS

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86203.1.
RefSeq	YP_153458.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3171313.
GenomeReviews	Gene locus AM018 in contig CP000030_GR.
KEGG	ama:AM018.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PBX6.
OMA	Q5PBX6. EFNNAYR.
Enzyme and pathway databases
BioCyc	AMAR234826:AM018-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_ANAMM

Accession

Primary (citable) accession number: Q5PBX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)