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Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Anaplasma marginale (strain St. Maries)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 1UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 1UniRule annotation
Short name:
GluRS 1UniRule annotation
Gene namesi
Name:gltX1UniRule annotation
Ordered Locus Names:AM039
OrganismiAnaplasma marginale (strain St. Maries)
Taxonomic identifieri234826 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001194921 – 441Glutamate--tRNA ligase 1Add BLAST441

Proteomic databases

PRIDEiQ5PBW1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi234826.AM039.

Structurei

3D structure databases

ProteinModelPortaliQ5PBW1.
SMRiQ5PBW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 17"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252721.
KOiK01885.
OMAiQMKETAK.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5PBW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITRFAPSPT GYMHIGNART ALICWLYARS RSGKLLLRID DTDLERSENR
60 70 80 90 100
YVEGIKNDLT WLAMDWDICF NQRSRISRYD EVFNSLMDAG AVYPCYETPE
110 120 130 140 150
ELELKRKMML KMGLPPIYDR SALNMTEQDQ KAYSGRKPYF RLKIGRDQAI
160 170 180 190 200
SWEDEIRGKV VFQAKNISDP ILRRTDGSYT YMFPSTVDDI DFEVTHIVRG
210 220 230 240 250
EDHVSNTAVQ IYIMGLLGAK IPSFAHLPLL RMGGSKMSKR VGGTEIFKMR
260 270 280 290 300
DMNLEPMAIN SYMARIGTSL PVEPHTNMRS LVDSFDIKLF NQAPIKFELE
310 320 330 340 350
DISKLNVRLL QKLPFAEVQD RLEACGIKCS EGFWYAVRDN INVISEVKGW
360 370 380 390 400
AEICGPGVTP VVDDVNRQLL QLASDLLPEG EPNDGTWKTW LQKIKECSGC
410 420 430 440
ETRDILLPLR LALTGVPKGP EFAKLLPLIG RVEILRRLRG A
Length:441
Mass (Da):50,239
Last modified:January 4, 2005 - v1
Checksum:i3CC9F08ABE6409A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000030 Genomic DNA. Translation: AAV86218.1.
RefSeqiWP_011114091.1. NZ_AFMU01000007.1.

Genome annotation databases

EnsemblBacteriaiAAV86218; AAV86218; AM039.
KEGGiama:AM039.
PATRICi20946191. VBIAnaMar46146_0036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000030 Genomic DNA. Translation: AAV86218.1.
RefSeqiWP_011114091.1. NZ_AFMU01000007.1.

3D structure databases

ProteinModelPortaliQ5PBW1.
SMRiQ5PBW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234826.AM039.

Proteomic databases

PRIDEiQ5PBW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV86218; AAV86218; AM039.
KEGGiama:AM039.
PATRICi20946191. VBIAnaMar46146_0036.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252721.
KOiK01885.
OMAiQMKETAK.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE1_ANAMM
AccessioniPrimary (citable) accession number: Q5PBW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 4, 2005
Last modified: February 15, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.