Q5PBV2 (SYFA_ANAMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	AM055

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000231960
Sites
	Metal binding	266	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PBV2 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 00020A4FF87B5D4D	FASTA	351	39,649
	10 20 30 40 50 60 MGMLVPLISK ISDLSEEAKA CVAACSSVEE LDEVRGRYIG RAGALTALLR QISTIQDMDE 70 80 90 100 110 120 RKAVGSAANA ACAALKLAIQ DRESQLAREQ LHSRLASERI DVTLPARPRT CGKIHPISGV 130 140 150 160 170 180 IREISSILSE LGFAVVHGPE LEDEFHVFDA LNTPEHHPAR AENDTFYMTK RLNGRRVVLR 190 200 210 220 230 240 THTSSMQIRA MESNPNPPIK IISPGRVYRN DWDATHSPVF HQVEGLFVDK HVTMGHLKYC 250 260 270 280 290 300 INYFLSRFFA RKVETRMRAS FFPFTEPSAE IDVKDRHQKW VEVLGCGMVH PAVLENVNID 310 320 330 340 350 PEKYRGFAFG MGVERMAMLK YGITDLRNFY SNKLEWLNHY GFCFTDILGR A

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: St. Maries.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000030 Genomic DNA. Translation: AAV86227.1.
RefSeq	YP_153482.1. NC_004842.2.
3D structure databases
ProteinModelPortal	Q5PBV2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PBV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3171485.
GenomeReviews	Gene locus AM055 in contig CP000030_GR.
KEGG	ama:AM055.
PATRIC	20946227. VBIAnaMar46146_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q5PBV2.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	AMAR234826:AM055-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ANAMM

Accession

Primary (citable) accession number: Q5PBV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program