Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q5PBK2 (DDL_ANAMM)

Last modified November 25, 2008. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase
Gene names
Name: ddl
Ordered Locus Names: AM205
OrganismAnaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]
Taxonomic identifier234826 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Cell wall formation By similarity.

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

CytoplasmBy similarity.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334D-alanine--D-alanine ligase
PRO_0000341052

Regions

Domain110 – 306197ATP-grasp
Nucleotide binding138 – 19053ATP By similarity

Sites

Metal binding2581Magnesium or manganese 1 By similarity
Metal binding2721Magnesium or manganese 1 By similarity
Metal binding2721Magnesium or manganese 2 By similarity
Metal binding2741Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5PBK2-1 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 223FB0FC1F3F40AF

FASTA33436,433
        10         20         30         40         50         60 
MPVSLARNAG MLSVAVLCGG SSPEREVSLA GGKRVADALG RLGYNATVLD LNRESVGQLL 

        70         80         90        100        110        120 
AMAPDLVYNA LHGVQGEDGC VSGLLDILGL ACTHSHVAAS SVGMDKVLTK HVLKSLGIDF 

       130        140        150        160        170        180 
PKFDVLTKEE LLSAKEVLPY PFVIKPVRGG STIGVHAIFS KSEYLDLSAH ADTLEDRMIV 

       190        200        210        220        230        240 
EEYVSGQEVQ TAVFLGRAIG TMELLFEGRI YSYDAKYVEG LCEHIFPANL PYDIYNLTLE 

       250        260        270        280        290        300 
WALKLHQCLG CKTLSRVDFR YDVANKALKL LEINTHPGMT VSSTLPEVLW LRCGLNFDHV 

       310        320        330 
VDLIVQDALG IDDSRRAYID ELMGKTVSRE PSHV 

« Hide

References

[1]"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins."
Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr.
Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000030 Genomic DNA. Translation: AAV86327.1.
RefSeqYP_153582.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3171882.
GenomeReviewsGene locus AM205 in contig CP000030_GR.
KEGGama:AM205.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ5PBK2.

Enzyme and pathway databases

BioCycAMAR234826:AM205-MON.

Family and domain databases

HAMAPMF_00047.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR013817. Pre-ATP_grasp.
[Graphical view]
Gene3DG3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ANAMM
AccessionPrimary (citable) accession number: Q5PBK2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 4, 2005
Last modified: November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents