Reviewed, UniProtKB/Swiss-Prot Q5PBH9 (MURD_ANAMM)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	AM243

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108956
Regions
	Nucleotide binding	102 – 108	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5PBH9-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: A49CF2AD535B6934	FASTA	438	47,178
	10 20 30 40 50 60 MGETGISTVC SLVQSGARVL AWDDDKCVLR KNRELCAGCE FVHPYEYRWD EISELVLSPG 70 80 90 100 110 120 VPHSRCNESW VVKLAKRAGC HITSDVEIFC RSRPARLIGV TGTNGKSTTV ELIGWILNRA 130 140 150 160 170 180 GIDSETGGNI GKGVLSLPQG KAAYILELSS YQLELMRGVN LDVGVILNIT PNHLDVYSNM 190 200 210 220 230 240 ADYIAAKGRI LEFSNLLVAN CGDSNTAHLC ENVGNMVEFS CGKKLARGVS VLDGRIYFQG 250 260 270 280 290 300 RVAEIGGLKI DSASNAENIA ASYAVAKSLG VSDEVIMASI REFPGLRHRN QVVAEVANVT 310 320 330 340 350 360 FVNDSKATTA ASVAKALVSR ENVYWIAGGK SKDGGIEPIL SLYANRIRKA FLVGEAADMF 370 380 390 400 410 420 AVSLQRARVE HVMCYDVYNA VQIAFAEAIA SGTKATVLLS PACSSLDQWK NFEERGDAFC 430 AAVAELVAPH KLCSTTTQ

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86350.1.
RefSeq	YP_153605.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PBH9.
Genome annotation databases
GeneID	3171617.
GenomeReviews	Gene locus AM243 in contig CP000030_GR.
KEGG	ama:AM243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PBH9.
OMA	DSKATNF.
Enzyme and pathway databases
BioCyc	AMAR234826:AM243-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ANAMM

Accession

Primary (citable) accession number: Q5PBH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)