Q5PBH2 (EFG_ANAMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	AM253

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Elongation factor G HAMAP MF_00054_B		PRO_0000091055
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PBH2 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E396318727D0C14E	FASTA	690	75,579
	10 20 30 40 50 60 MSSKGDLSRC RNIGIMAHID AGKTTTTERI LFYTGKQNRI GEVHEGAASM DWMEQERERG 70 80 90 100 110 120 ITITSAATTC FWNDCRINII DTPGHVDFTI EVERSLRVLD GAVAVFDGVA GVEPQSETVW 130 140 150 160 170 180 RQADKYDVPR ICFVNKMDRI GADFYACVDM IKDRLGAVPL VLQLPIGVDK SFVGVVDLVE 190 200 210 220 230 240 MRSITWEEDS LGAKFNYGEI PSDLMEKAQD YRARLIESAV EMNDEAMNLY LDGGEISVPL 250 260 270 280 290 300 LKSCIRSGVI GAKFVPVLCG SAFKNKGVQP LLDAVVDFLP SPSDIPTIEG ASASDPQKAV 310 320 330 340 350 360 TIKSSVDDKF VALAFKVMVD RFVGSLTFIR VYSGKLTGKS VVLNSAKGVT ESVGRILRMH 370 380 390 400 410 420 ANNREDISEI QAGDIAALAG LKKTTTGDTL CDQNFPVVLE KMDFPESVME IAVEPVSTAD 430 440 450 460 470 480 QEKMGTALSR LVAEDPSLKV CVNSESGQTI LKGMGELHLE IIVDRMKREF GVEASVGAPQ 490 500 510 520 530 540 VAYRETITKS AEIEYVHKKQ TGGAGQFAKV NILFEPLPPG SGFEFENKIT CGAIPKEYIP 550 560 570 580 590 600 GVQSGLELVK ETGMIAGFPV IDFKATLFDG AFHEVDSSPL AFELAAKGAF REMANKAGPV 610 620 630 640 650 660 LLEPIMRVEI ITPDEYMGDV IGDVNSRRGR VAEMQDRHNA KLITAFIPLG KMFGYVKDLR 670 680 690 SMSQGRAQYS MYFARYERVP ENAVDNVMKK

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: St. Maries.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000030 Genomic DNA. Translation: AAV86357.1.
RefSeq	YP_153612.1. NC_004842.2.
3D structure databases
ProteinModelPortal	Q5PBH2.
SMR	Q5PBH2. Positions 5-689.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PBH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3171791.
GenomeReviews	Gene locus AM253 in contig CP000030_GR.
KEGG	ama:AM253.
PATRIC	20946565. VBIAnaMar46146_0218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
PhylomeDB	Q5PBH2.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	AMAR234826:AM253-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_ANAMM

Accession

Primary (citable) accession number: Q5PBH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program