Reviewed, UniProtKB/Swiss-Prot Q5PBF1 (MURE_ANAMM)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	AM282

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012334
Regions
	Nucleotide binding	99 – 105	7	ATP Potential
	Region	143 – 144	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	391 – 394	4	Meso-diaminopimelate binding By similarity
	Motif	391 – 394	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	21	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	170	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	176	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	178	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	367	1	Meso-diaminopimelate By similarity
	Binding site	440	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	444	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	210	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PBF1-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: F0CEA09ED8DD6DAD	FASTA	472	51,235
	10 20 30 40 50 60 MNIDSIVREL IGQASGITAD SRSVKPGFIF VCLSEAAKGF VPDAVKAGAK FIVAGNAGGL 70 80 90 100 110 120 PHVDHSQIII CPMQHDLYYK LASEFYHASQ PEFVAVVTGT NGKTSVADFC RQIWCLTGHT 130 140 150 160 170 180 GASMGTLGAI VGNTTLPSST SFTTPDAVEL HKTLSTLRDL QVKYLCMEAS SHGMAQKRLY 190 200 210 220 230 240 GVKASAAAFT NLSGDHLDYH GSMEKYWATK QKLFSEVLSC EGCAVLNADS EQYGELRELA 250 260 270 280 290 300 AGRNIITYGV DTGDVKLARL ERNTQGHNIT VKVCGEVIYD GAFPVLGKFQ ISNLLCALAI 310 320 330 340 350 360 VAASGSGHRD VPIDRLASPR GRMELIGDRI IIDYAHTSDA LKHALTSLKW HGFPQKTVLV 370 380 390 400 410 420 FGCGGDRDRE KRKTMGMVAS QYADVVIVTD DNPRSENPET IRKEIMLHCE NAIEIPDRGD 430 440 450 460 470 AIHYAVNFAI RNNYVLLIAG KGHETTQQIA GKSVEFNDGT AVRRCLSEMQ YA

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000030 Genomic DNA. Translation: AAV86378.1.
RefSeq	YP_153633.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8C based on UniProtKB P22188.
SMR	Q5PBF1. Positions 14-469.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PBF1.
Genome annotation databases
GeneID	3171129.
GenomeReviews	Gene locus AM282 in contig CP000030_GR.
KEGG	ama:AM282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HBG602753.
OMA	HTPDGIE.
Enzyme and pathway databases
BioCyc	AMAR234826:AM282-MONOMER.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_ANAMM

Accession

Primary (citable) accession number: Q5PBF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)