Reviewed, UniProtKB/Swiss-Prot Q5PB64 (DAPA_ANAMM)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	AM407

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000340934
Regions
	Region	49 – 50	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	107	1	Pyruvate By similarity
	Site	134	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PB64-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 3DEE72847BB9800A	FASTA	301	32,385
	10 20 30 40 50 60 MKLNGVFTAL ATPFRDDLSL DERALASFVD WQISSGISGI VPCGTTGESA TLNFEEYCTV 70 80 90 100 110 120 VRLCIETARG RILVIAGAGS HCTTETISRA LFVQSAGADA ALIVVPYYNR PSDEGVYQHF 130 140 150 160 170 180 RAVHDATNIP IVLYNVPQRT AIDISNDTIR RIAELPRVVG IKDCTGAERV AALKAILPEK 190 200 210 220 230 240 VAILSGEDET ALASYMNGGS GCISVVSNVA PKMAVELYRL HALGKINMAK QVSGNLAALS 250 260 270 280 290 300 RVLFIEPSPS PTKYALSLMG KMRPKVRLPL VELTSSGQTA VKNVLETLDL LRQQKAMHSQ L

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86465.1.
RefSeq	YP_153720.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PB64.
Genome annotation databases
GeneID	3171207.
GenomeReviews	Gene locus AM407 in contig CP000030_GR.
KEGG	ama:AM407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PB64.
OMA	CISAVSN.
Enzyme and pathway databases
BioCyc	AMAR234826:AM407-MON.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_ANAMM

Accession

Primary (citable) accession number: Q5PB64

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)