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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Anaplasma marginale (strain St. Maries)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:AM446
OrganismiAnaplasma marginale (strain St. Maries)
Taxonomic identifieri234826 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829Leucine--tRNA ligasePRO_0000334727Add
BLAST

Proteomic databases

PRIDEiQ5PB38.

Interactioni

Protein-protein interaction databases

STRINGi234826.AM446.

Structurei

3D structure databases

ProteinModelPortaliQ5PB38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5PB38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFFGMGYNF KLVEQEIQKR WDFKIAGNDG INCYVLGMFP YPSGNIHMGH
60 70 80 90 100
IRNYTIGDVI ARYKRAHGLK VLHPIGWDAF GLPAENAALS YGVNPAVWTE
110 120 130 140 150
RNIESMRKQL KSIGISYNWD RELATCKEDY YKHEQAFFLD FLQQGLAYRK
160 170 180 190 200
ESWVNWDPVD NTVLANEQVV DGRGWRSGAK IERRKLSQWF LKITDFADSL
210 220 230 240 250
LDGLKTLDGW PEKVKLMQER WIGKTEGVIL EFATSCGQKL EVFSTMPHML
260 270 280 290 300
FGASFCAVSA EHPILRHVTD EAFSARVRGI IECSGDEEKQ KIGADTGLFA
310 320 330 340 350
THPLLDRKLP IYAANYVLSE YGTGAVFGCP AHDQRDFEFA VAHGLDIYQV
360 370 380 390 400
VFPDDGVQYD LQKEAYSGDG TYRNSEFLNG LRVDAARNAM IQKLESMGGC
410 420 430 440 450
RRVTNYRLHD WGISRQRYWG CPIPVVHCEK CGIVPVDRQD LPISLPEEVD
460 470 480 490 500
FSRGGNPLDH HPTWKHVQCP KCQSSAQRET DTFDTFFESS WYFAAFCSEQ
510 520 530 540 550
GGINAADCNK LLPVDYYIGG VEHAVLHLLY ARFFCRALKK CGHLAVEEPF
560 570 580 590 600
RNLITQGMVC HSVYRDAAGN YLFPEDAQRM IRDGEAVQRG KVEKMSKSKK
610 620 630 640 650
NVVDPSHIIK KYGADTVRLF MLSDTPPERD IEWSDVGVEG AWRYLERLWR
660 670 680 690 700
LFEDNSSIGA DFDTADVKAE DRVYLSGIHK LLRGLSADME HCRLNCAVAK
710 720 730 740 750
FREMSNTVFE MVKCGVSQQV INESVCILLR VMEPFIPHIA EKLWERIGGE
760 770 780 790 800
GMLCNRQWPS AREDLLTEDL VTIAVQVNGK LCSTLKVGAQ CDGEEVKAEA
810 820
LKVAQRKLGD KEVRNIYFVP GRVVNIVTK
Length:829
Mass (Da):94,109
Last modified:January 4, 2005 - v1
Checksum:iE5881D97AB91F66E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000030 Genomic DNA. Translation: AAV86492.1.

Genome annotation databases

EnsemblBacteriaiAAV86492; AAV86492; AM446.
KEGGiama:AM446.
PATRICi20946921. VBIAnaMar46146_0386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000030 Genomic DNA. Translation: AAV86492.1.

3D structure databases

ProteinModelPortaliQ5PB38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234826.AM446.

Proteomic databases

PRIDEiQ5PB38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV86492; AAV86492; AM446.
KEGGiama:AM446.
PATRICi20946921. VBIAnaMar46146_0386.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins."
    Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr.
    Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: St. Maries.

Entry informationi

Entry nameiSYL_ANAMM
AccessioniPrimary (citable) accession number: Q5PB38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 4, 2005
Last modified: July 22, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.