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Q5PB38 (SYL_ANAMM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:AM446
OrganismAnaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]
Taxonomic identifier234826 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334727

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif594 – 5985"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5971ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5PB38 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: E5881D97AB91F66E

FASTA82994,109
        10         20         30         40         50         60 
MEFFGMGYNF KLVEQEIQKR WDFKIAGNDG INCYVLGMFP YPSGNIHMGH IRNYTIGDVI 

        70         80         90        100        110        120 
ARYKRAHGLK VLHPIGWDAF GLPAENAALS YGVNPAVWTE RNIESMRKQL KSIGISYNWD 

       130        140        150        160        170        180 
RELATCKEDY YKHEQAFFLD FLQQGLAYRK ESWVNWDPVD NTVLANEQVV DGRGWRSGAK 

       190        200        210        220        230        240 
IERRKLSQWF LKITDFADSL LDGLKTLDGW PEKVKLMQER WIGKTEGVIL EFATSCGQKL 

       250        260        270        280        290        300 
EVFSTMPHML FGASFCAVSA EHPILRHVTD EAFSARVRGI IECSGDEEKQ KIGADTGLFA 

       310        320        330        340        350        360 
THPLLDRKLP IYAANYVLSE YGTGAVFGCP AHDQRDFEFA VAHGLDIYQV VFPDDGVQYD 

       370        380        390        400        410        420 
LQKEAYSGDG TYRNSEFLNG LRVDAARNAM IQKLESMGGC RRVTNYRLHD WGISRQRYWG 

       430        440        450        460        470        480 
CPIPVVHCEK CGIVPVDRQD LPISLPEEVD FSRGGNPLDH HPTWKHVQCP KCQSSAQRET 

       490        500        510        520        530        540 
DTFDTFFESS WYFAAFCSEQ GGINAADCNK LLPVDYYIGG VEHAVLHLLY ARFFCRALKK 

       550        560        570        580        590        600 
CGHLAVEEPF RNLITQGMVC HSVYRDAAGN YLFPEDAQRM IRDGEAVQRG KVEKMSKSKK 

       610        620        630        640        650        660 
NVVDPSHIIK KYGADTVRLF MLSDTPPERD IEWSDVGVEG AWRYLERLWR LFEDNSSIGA 

       670        680        690        700        710        720 
DFDTADVKAE DRVYLSGIHK LLRGLSADME HCRLNCAVAK FREMSNTVFE MVKCGVSQQV 

       730        740        750        760        770        780 
INESVCILLR VMEPFIPHIA EKLWERIGGE GMLCNRQWPS AREDLLTEDL VTIAVQVNGK 

       790        800        810        820 
LCSTLKVGAQ CDGEEVKAEA LKVAQRKLGD KEVRNIYFVP GRVVNIVTK 

« Hide

References

[1]"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins."
Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr.
Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: St. Maries.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000030 Genomic DNA. Translation: AAV86492.1.
RefSeqYP_153747.1. NC_004842.2.

3D structure databases

ProteinModelPortalQ5PB38.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING234826.AM446.

Proteomic databases

PRIDEQ5PB38.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV86492; AAV86492; AM446.
GeneID3171345.
KEGGama:AM446.
PATRIC20946921. VBIAnaMar46146_0386.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ANAMM
AccessionPrimary (citable) accession number: Q5PB38
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries