Reviewed, UniProtKB/Swiss-Prot Q5PAZ5 (SYP_ANAMM)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AM507

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570
Subunit structure	Homodimer By similarity. HAMAP MF_01570
Subcellular location	Cytoplasm By similarity HAMAP MF_01570.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Prolyl-tRNA synthetase HAMAP MF_01570		PRO_0000248887

Sequences

	Sequence		Length	Mass (Da)
	Q5PAZ5-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: C778F95246013B1D	FASTA	425	47,664
	10 20 30 40 50 60 MRLSECYVPT MKDVSSDVVV ASHKYSLRAG LVRQNASGLY TWLPLGLKVL RTIERIVREE 70 80 90 100 110 120 MDSSGFLEIL MPSVQPADLW RESLRYDSYG PEMLRMQDRS GREMVFGPTH EEAISDVVRS 130 140 150 160 170 180 SLKSYRDLPI NLYQIQWKFR DELRPRHGIM RGREFLMKDA YSFDVDFEGV MRSYENVFSA 190 200 210 220 230 240 YFRIFRRLGL VPIAAKADSG AIGGSISHEF HVLVPTGEST VYHDKKALDL SKNDYCTTEE 250 260 270 280 290 300 IASVYAATED AHDPQNCGVD PNDLQVSKGI EVAHVFYLGD RYSAPMNVKF HDKDGNSAHA 310 320 330 340 350 360 LMGCYGIGIS RLVAAIIEVF HDDVGIRWPE SIAPFKVGIV NLLTTNEDCK TTAETIYAAL 370 380 390 400 410 420 ADSSLYDDST DSPGVKLARM DLLGMPWQVI IGNSFVKDKV VELKNRANGA TERCTVDALI ARMQA

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000030 Genomic DNA. Translation: AAV86535.1.
RefSeq	YP_153790.1.
3D structure databases
SMR	Q5PAZ5. Positions 1-422.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PAZ5.
Genome annotation databases
GeneID	3171578.
GenomeReviews	Gene locus AM507 in contig CP000030_GR.
KEGG	ama:AM507.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
PhylomeDB	Q5PAZ5.
Enzyme and pathway databases
BioCyc	AMAR234826:AM507-MONOMER.
Family and domain databases
HAMAP	MF_01570. Pro_tRNA_synth_type2. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ANAMM

Accession

Primary (citable) accession number: Q5PAZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)