Reviewed, UniProtKB/Swiss-Prot Q5PAV3 (MDH_ANAMM)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AM564

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Malate dehydrogenase HAMAP MF_00487		PRO_0000113420
Regions
	Nucleotide binding	13 – 18	6	NAD By similarity
	Nucleotide binding	123 – 125	3	NAD By similarity
Sites
	Active site	180	1	Proton acceptor By similarity
	Binding site	38	1	NAD By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	100	1	NAD By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PAV3-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 65C4ED86C35C6D0B	FASTA	317	33,379
	10 20 30 40 50 60 MRSSRSAKVS LVGAGNIGGA LAHMLGASQV VKELVLVDVA GGMTEGKVLD VGQALALLGS 70 80 90 100 110 120 DVYITGGSDY AAIEHSDAVV VTAGIPRKEG MSREDLLNTN AAVVRNIAEN IAKYSPGALV 130 140 150 160 170 180 IVVTNPLDAM VWCMYKYSGL PANRVVGMAG VLDSARFSFF LARHMNVSVS SVSAMVLGGH 190 200 210 220 230 240 GDLMLPLLRY STVGGVPVES LIESGRLNRD DIAAIVERTR KGGEEIVKLL KTGSAYCAPA 250 260 270 280 290 300 ASCAHMLESY VRDKRSIMPC SAYLDGQYGV RDLFVGVPVI IGEKGVEEVV EFPLTAEEQA 310 VFDQSVELIR GSVSAIS

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86577.1.
RefSeq	YP_153832.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3171816.
GenomeReviews	Gene locus AM564 in contig CP000030_GR.
KEGG	ama:AM564.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PAV3.
OMA	Q5PAV3. CINIAPS.
Enzyme and pathway databases
BioCyc	AMAR234826:AM564-MON.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_NAD-dep_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_ANAMM

Accession

Primary (citable) accession number: Q5PAV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)