Q5PAV3 (MDH_ANAMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AM564

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Malate dehydrogenase HAMAP MF_00487		PRO_0000113420
Regions
	Nucleotide binding	13 – 18	6	NAD By similarity
	Nucleotide binding	123 – 125	3	NAD By similarity
Sites
	Active site	180	1	Proton acceptor By similarity
	Binding site	38	1	NAD By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	100	1	NAD By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PAV3 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 65C4ED86C35C6D0B	FASTA	317	33,379
	10 20 30 40 50 60 MRSSRSAKVS LVGAGNIGGA LAHMLGASQV VKELVLVDVA GGMTEGKVLD VGQALALLGS 70 80 90 100 110 120 DVYITGGSDY AAIEHSDAVV VTAGIPRKEG MSREDLLNTN AAVVRNIAEN IAKYSPGALV 130 140 150 160 170 180 IVVTNPLDAM VWCMYKYSGL PANRVVGMAG VLDSARFSFF LARHMNVSVS SVSAMVLGGH 190 200 210 220 230 240 GDLMLPLLRY STVGGVPVES LIESGRLNRD DIAAIVERTR KGGEEIVKLL KTGSAYCAPA 250 260 270 280 290 300 ASCAHMLESY VRDKRSIMPC SAYLDGQYGV RDLFVGVPVI IGEKGVEEVV EFPLTAEEQA 310 VFDQSVELIR GSVSAIS

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: St. Maries.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000030 Genomic DNA. Translation: AAV86577.1.
RefSeq	YP_153832.1. NC_004842.2.
3D structure databases
ProteinModelPortal	Q5PAV3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PAV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3171816.
GenomeReviews	Gene locus AM564 in contig CP000030_GR.
KEGG	ama:AM564.
PATRIC	20947151. VBIAnaMar46146_0496.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	ANCAVRI.
PhylomeDB	Q5PAV3.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	AMAR234826:AM564-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_ANAMM

Accession

Primary (citable) accession number: Q5PAV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program