Reviewed, UniProtKB/Swiss-Prot Q5P9T3 (PANC_ANAMM)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase
      Short name=PS
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase
    Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	AM1089

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity.
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Pantothenate synthetase HAMAP MF_00158		PRO_0000305391
Regions
	Nucleotide binding	28 – 35	8	ATP By similarity
	Nucleotide binding	145 – 148	4	ATP By similarity
	Nucleotide binding	182 – 185	4	ATP By similarity
Sites
	Active site	35	1	Proton donor By similarity
	Binding site	59	1	Beta-alanine By similarity
	Binding site	59	1	Pantoate By similarity
	Binding site	174	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P9T3-1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 29ADC2883893FD5E	FASTA	277	31,057
	10 20 30 40 50 60 MEIVRDVESV RAVLRPFTNR KIGLVPTMGA LHSGHLSLVH EMKKHADVVI VSIFVNPLQF 70 80 90 100 110 120 SPGEDYEKYP RCEEVDCEKC ASAGVDVVYI PSAEGMYPDG FSTSVDIGPM ARELCGASRQ 130 140 150 160 170 180 NFINGIMVVL IKLVMQTNAH CMILGEKDYQ MLHLTRQLFR DLNIGVDVLQ GNTVRSAEGL 190 200 210 220 230 240 ALSSRHQYLS SAEITKANFL YGFLLEVGQQ LSDDPHGQQE IIARGKLRLE QEGFEVDYLE 250 260 270 VRDNNTLEHM QTFRKPARVF LAVYLGNCRL IDNVLLA

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86947.1. Different initiation.
RefSeq	YP_154202.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P9T3.
Genome annotation databases
GeneID	3171235.
GenomeReviews	Gene locus AM1089 in contig CP000030_GR.
KEGG	ama:AM1089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P9T3.
OMA	ASSKENH.
Enzyme and pathway databases
BioCyc	AMAR234826:AM1089-MON.
Family and domain databases
HAMAP	MF_00158. [Tree]
InterPro	IPR004821. Cyt_trans_rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ANAMM

Accession

Primary (citable) accession number: Q5P9T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	November 3, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)