Q5P8W0 (AROD_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	AZOSEA01270
ORF Names:	ebA234

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325517
Sites
	Active site	148	1	Proton acceptor By similarity
	Active site	175	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P8W0 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 5BA53ADD0ACB474D	FASTA	260	28,270
	10 20 30 40 50 60 MAMTRSLEVK GQLVAGGKEP LICAPLVGRD EAAVLRELEV IVAKRPDLLE WRVDFYSGIS 70 80 90 100 110 120 DVERVVELAK EIRRSSAGIP VIFTRRSTRE GGEPIGLSED EVLSMYRAVA RSGAVDFLDY 130 140 150 160 170 180 ELSSQPEHFE QAVALARETD TKLIASFHDF GKTPPVDEIV AKLVAMEEAG ADIAKVAVMP 190 200 210 220 230 240 QGLEDVLTLL QATLEAQKKI RLPVISMSMG AYGSLSRLFG WVFGSSVSFA VGQQASAPGQ 250 260 VPIEDLRTVL EVLQRSLKGE

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI06249.1.
RefSeq	YP_157150.1. NC_006513.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQN based on UniProtKB P24670.
ProteinModelPortal	Q5P8W0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P8W0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3181092.
GenomeReviews	Gene locus AZOSEA01270 in contig CR555306_GR.
KEGG	eba:ebA234.
NMPDR	fig|76114.4.peg.208.
PATRIC	20966297. VBIAroAro98752_0127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	ASP76114:EBA234-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_AROAE

Accession

Primary (citable) accession number: Q5P8W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program