Reviewed, UniProtKB/Swiss-Prot Q5P8J4 (ILVD1_AZOSE)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	AZOSEA02430
ORF Names:	ebA478

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225364
Sites
	Metal binding	130	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	202	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5P8J4-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: B73DEA2A4BC623C7	FASTA	567	59,808
	10 20 30 40 50 60 MSDPTRPARI DERSRNVTEG VMRAPNRSMY YAMGYRETDF GKPMVGVASA HSTITPCNSG 70 80 90 100 110 120 LQPLADTVAA ALKEAGANPQ LFGTPTVSDG IGMGTEGMKY SLVSREVIAD SIETCVNGLW 130 140 150 160 170 180 QDGVVVIGGC DKNMPGGMMA LVRTNVPGIY VYGGTIKPGH YKGRDLNIVS VFEAVGEFIA 190 200 210 220 230 240 GQLDPVDFKE IEKRACPGSG SCGGMYTANT MSAAFEALGM SLPYSSTMAN EDAEKLASAA 250 260 270 280 290 300 ESARVLVEAI KRGLRPRDIV TREAIENAVS VIMATGGSTN AVLHFLAIAH AAEVPWTIDD 310 320 330 340 350 360 FERIRRRVPV IVDMKPSGRY LATDLHQAGG IPQVMKLLLD AGLLHGACVT ITGQTIAEVL 370 380 390 400 410 420 ENVPAAPRAD QGVIRTLSDP LYAEGHLAIL RGNLSPEGCV AKISGLKNPA ITGPARVFDS 430 440 450 460 470 480 EDDAMAAIMA RRIVEGDVVV IRYEGPKGGP GMREMLAPTS ALVGQGLGES VGLITDGRFS 490 500 510 520 530 540 GGTWGMVVGH VSPEAFVGGP IALVREGDSV TIDAHRQLVQ LNVGDEELAR RAADWTPPSP 550 560 RYTRGVLAKF AKFASSASKG AVTDLDL

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06365.1.
RefSeq	YP_157266.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P8J4.
Genome annotation databases
GeneID	3182262.
GenomeReviews	Gene locus AZOSEA02430 in contig CR555306_GR.
KEGG	eba:ebA478.
NMPDR	fig|76114.4.peg.795.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P8J4.
OMA	QVMKILL.
Enzyme and pathway databases
BioCyc	ASP76114:EBA478-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_AZOSE

Accession

Primary (citable) accession number: Q5P8J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)