Reviewed, UniProtKB/Swiss-Prot Q5P811 (MASZ_AZOSE)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	AZOSEA04280
ORF Names:	ebA819

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G HAMAP MF_00641		PRO_1000056892
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	633	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P811-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 521DE6ACFB25F40E	FASTA	725	78,732
	10 20 30 40 50 60 MTERIQCNQL QVDAALHRFI ENEALPGTGL EAAAFWKGFS ELANELAPKN RALLAERDRL 70 80 90 100 110 120 QAEIDTWHRA HPGPIQDMPA YRAFLESIGY LQPVPAPFKI TTANVDSEIS EQAGPQLVVP 130 140 150 160 170 180 IMNARYALNA ANARWGSLYD ALYGTNAIPS DGGAEAGSSY NPVRGARVIA FARNLLDQAA 190 200 210 220 230 240 PLAQGSHDKT KAYAVAGGKL IVTLLDGSQT GLADPAKFAG FQGDAAAPSA VLLKNNGLHL 250 260 270 280 290 300 EIQFDRKHPI GKTDAAGIKD VLVEAAVTTI MDCEDSTAAV DAEDKVVVYR SWLGLITGTL 310 320 330 340 350 360 VETFDKGGKT VERKMNPDRE YTAPNGSALR LHGRSLLFIR HVGHLMTNPA ILLADGAEIP 370 380 390 400 410 420 EGIMDGVVTT LISMRDLKLK RNSRTDSMYI VKPKMHGPAE VAFASELFGR VEQLLGLPAD 430 440 450 460 470 480 TMKVGIMDEE RRTSVNLAGC IKAAESRVAF INTGFLDRTG DEMHTAMEAG PMIRKGDMKG 490 500 510 520 530 540 SAWIQAYERQ NVLVGLDCGL RGRAQIGKGM WAMPDLMAEM LEQKIGHPKA GATTAWVPSP 550 560 570 580 590 600 TGATLHALHY HQVDVQSVQQ EMEKISLAAE RDTLLDNLLT VPVAASANWS ATEIQQELDN 610 620 630 640 650 660 NAQGILGYVV RWIDQGVGCS KVPDINNIGL MEDRATLRIS SQHIANWLRH GVTNEAQVLE 670 680 690 700 710 720 TMKRMAAVVD QQNAGDPLYR PMAPDFDQSV AFQAAKDLVF KGCAQPSGYT EPLLHRWRQV AKARG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI06550.1.
RefSeq	YP_157451.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB P0A5J4.
SMR	Q5P811. Positions 1-718.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P811.
Genome annotation databases
GeneID	3179182.
GenomeReviews	Gene locus AZOSEA04280 in contig CR555306_GR.
KEGG	eba:ebA819.
NMPDR	fig|76114.4.peg.1177.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HBG350005.
OMA	SQFIENE.
Enzyme and pathway databases
BioCyc	ASP76114:EBA819-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_AZOSE

Accession

Primary (citable) accession number: Q5P811

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)