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Q5P811

- MASZ_AROAE

UniProt

Q5P811 - MASZ_AROAE

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Protein
Malate synthase G
Gene
glcB, AZOSEA04280, ebA819
Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei276 – 2761Acetyl-CoA By similarity
Binding sitei313 – 3131Acetyl-CoA By similarity
Active sitei340 – 3401Proton acceptor By similarity
Binding sitei340 – 3401Glyoxylate By similarity
Metal bindingi429 – 4291Magnesium By similarity
Binding sitei429 – 4291Glyoxylate By similarity
Metal bindingi457 – 4571Magnesium By similarity
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei633 – 6331Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAARO76114:GJTA-434-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:AZOSEA04280
ORF Names:ebA819
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GUniRule annotation
PRO_1000056892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei619 – 6191Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA819.

Structurei

3D structure databases

ProteinModelPortaliQ5P811.
SMRiQ5P811. Positions 1-718.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarity
Regioni454 – 4574Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5P811-1 [UniParc]FASTAAdd to Basket

« Hide

MTERIQCNQL QVDAALHRFI ENEALPGTGL EAAAFWKGFS ELANELAPKN    50
RALLAERDRL QAEIDTWHRA HPGPIQDMPA YRAFLESIGY LQPVPAPFKI 100
TTANVDSEIS EQAGPQLVVP IMNARYALNA ANARWGSLYD ALYGTNAIPS 150
DGGAEAGSSY NPVRGARVIA FARNLLDQAA PLAQGSHDKT KAYAVAGGKL 200
IVTLLDGSQT GLADPAKFAG FQGDAAAPSA VLLKNNGLHL EIQFDRKHPI 250
GKTDAAGIKD VLVEAAVTTI MDCEDSTAAV DAEDKVVVYR SWLGLITGTL 300
VETFDKGGKT VERKMNPDRE YTAPNGSALR LHGRSLLFIR HVGHLMTNPA 350
ILLADGAEIP EGIMDGVVTT LISMRDLKLK RNSRTDSMYI VKPKMHGPAE 400
VAFASELFGR VEQLLGLPAD TMKVGIMDEE RRTSVNLAGC IKAAESRVAF 450
INTGFLDRTG DEMHTAMEAG PMIRKGDMKG SAWIQAYERQ NVLVGLDCGL 500
RGRAQIGKGM WAMPDLMAEM LEQKIGHPKA GATTAWVPSP TGATLHALHY 550
HQVDVQSVQQ EMEKISLAAE RDTLLDNLLT VPVAASANWS ATEIQQELDN 600
NAQGILGYVV RWIDQGVGCS KVPDINNIGL MEDRATLRIS SQHIANWLRH 650
GVTNEAQVLE TMKRMAAVVD QQNAGDPLYR PMAPDFDQSV AFQAAKDLVF 700
KGCAQPSGYT EPLLHRWRQV AKARG 725
Length:725
Mass (Da):78,732
Last modified:January 4, 2005 - v1
Checksum:i521DE6ACFB25F40E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI06550.1.
RefSeqiYP_157451.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI06550; CAI06550; ebA819.
GeneIDi3179182.
KEGGieba:ebA819.
PATRICi20966895. VBIAroAro98752_0421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI06550.1 .
RefSeqi YP_157451.1. NC_006513.1.

3D structure databases

ProteinModelPortali Q5P811.
SMRi Q5P811. Positions 1-718.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76114.ebA819.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI06550 ; CAI06550 ; ebA819 .
GeneIDi 3179182.
KEGGi eba:ebA819.
PATRICi 20966895. VBIAroAro98752_0421.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci AARO76114:GJTA-434-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiMASZ_AROAE
AccessioniPrimary (citable) accession number: Q5P811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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