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Q5P811

- MASZ_AROAE

UniProt

Q5P811 - MASZ_AROAE

Protein

Malate synthase G

Gene

glcB

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei633 – 6331Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciAARO76114:GJTA-434-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:AZOSEA04280
    ORF Names:ebA819
    OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
    Taxonomic identifieri76114 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
    ProteomesiUP000006552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase GPRO_1000056892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei619 – 6191Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi76114.ebA819.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5P811.
    SMRiQ5P811. Positions 1-718.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5P811-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTERIQCNQL QVDAALHRFI ENEALPGTGL EAAAFWKGFS ELANELAPKN    50
    RALLAERDRL QAEIDTWHRA HPGPIQDMPA YRAFLESIGY LQPVPAPFKI 100
    TTANVDSEIS EQAGPQLVVP IMNARYALNA ANARWGSLYD ALYGTNAIPS 150
    DGGAEAGSSY NPVRGARVIA FARNLLDQAA PLAQGSHDKT KAYAVAGGKL 200
    IVTLLDGSQT GLADPAKFAG FQGDAAAPSA VLLKNNGLHL EIQFDRKHPI 250
    GKTDAAGIKD VLVEAAVTTI MDCEDSTAAV DAEDKVVVYR SWLGLITGTL 300
    VETFDKGGKT VERKMNPDRE YTAPNGSALR LHGRSLLFIR HVGHLMTNPA 350
    ILLADGAEIP EGIMDGVVTT LISMRDLKLK RNSRTDSMYI VKPKMHGPAE 400
    VAFASELFGR VEQLLGLPAD TMKVGIMDEE RRTSVNLAGC IKAAESRVAF 450
    INTGFLDRTG DEMHTAMEAG PMIRKGDMKG SAWIQAYERQ NVLVGLDCGL 500
    RGRAQIGKGM WAMPDLMAEM LEQKIGHPKA GATTAWVPSP TGATLHALHY 550
    HQVDVQSVQQ EMEKISLAAE RDTLLDNLLT VPVAASANWS ATEIQQELDN 600
    NAQGILGYVV RWIDQGVGCS KVPDINNIGL MEDRATLRIS SQHIANWLRH 650
    GVTNEAQVLE TMKRMAAVVD QQNAGDPLYR PMAPDFDQSV AFQAAKDLVF 700
    KGCAQPSGYT EPLLHRWRQV AKARG 725
    Length:725
    Mass (Da):78,732
    Last modified:January 4, 2005 - v1
    Checksum:i521DE6ACFB25F40E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI06550.1.
    RefSeqiYP_157451.1. NC_006513.1.

    Genome annotation databases

    EnsemblBacteriaiCAI06550; CAI06550; ebA819.
    GeneIDi3179182.
    KEGGieba:ebA819.
    PATRICi20966895. VBIAroAro98752_0421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI06550.1 .
    RefSeqi YP_157451.1. NC_006513.1.

    3D structure databases

    ProteinModelPortali Q5P811.
    SMRi Q5P811. Positions 1-718.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 76114.ebA819.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI06550 ; CAI06550 ; ebA819 .
    GeneIDi 3179182.
    KEGGi eba:ebA819.
    PATRICi 20966895. VBIAroAro98752_0421.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci AARO76114:GJTA-434-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
      Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
      Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EbN1.

    Entry informationi

    Entry nameiMASZ_AROAE
    AccessioniPrimary (citable) accession number: Q5P811
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3