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Protein

Malate synthase G

Gene

glcB

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei633Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciAARO76114:G1GHY-439-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:AZOSEA04280
ORF Names:ebA819
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
Proteomesi
  • UP000006552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000568921 – 725Malate synthase GAdd BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei619Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiQ5P811

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA819

Structurei

3D structure databases

ProteinModelPortaliQ5P811
SMRiQ5P811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q5P811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERIQCNQL QVDAALHRFI ENEALPGTGL EAAAFWKGFS ELANELAPKN
60 70 80 90 100
RALLAERDRL QAEIDTWHRA HPGPIQDMPA YRAFLESIGY LQPVPAPFKI
110 120 130 140 150
TTANVDSEIS EQAGPQLVVP IMNARYALNA ANARWGSLYD ALYGTNAIPS
160 170 180 190 200
DGGAEAGSSY NPVRGARVIA FARNLLDQAA PLAQGSHDKT KAYAVAGGKL
210 220 230 240 250
IVTLLDGSQT GLADPAKFAG FQGDAAAPSA VLLKNNGLHL EIQFDRKHPI
260 270 280 290 300
GKTDAAGIKD VLVEAAVTTI MDCEDSTAAV DAEDKVVVYR SWLGLITGTL
310 320 330 340 350
VETFDKGGKT VERKMNPDRE YTAPNGSALR LHGRSLLFIR HVGHLMTNPA
360 370 380 390 400
ILLADGAEIP EGIMDGVVTT LISMRDLKLK RNSRTDSMYI VKPKMHGPAE
410 420 430 440 450
VAFASELFGR VEQLLGLPAD TMKVGIMDEE RRTSVNLAGC IKAAESRVAF
460 470 480 490 500
INTGFLDRTG DEMHTAMEAG PMIRKGDMKG SAWIQAYERQ NVLVGLDCGL
510 520 530 540 550
RGRAQIGKGM WAMPDLMAEM LEQKIGHPKA GATTAWVPSP TGATLHALHY
560 570 580 590 600
HQVDVQSVQQ EMEKISLAAE RDTLLDNLLT VPVAASANWS ATEIQQELDN
610 620 630 640 650
NAQGILGYVV RWIDQGVGCS KVPDINNIGL MEDRATLRIS SQHIANWLRH
660 670 680 690 700
GVTNEAQVLE TMKRMAAVVD QQNAGDPLYR PMAPDFDQSV AFQAAKDLVF
710 720
KGCAQPSGYT EPLLHRWRQV AKARG
Length:725
Mass (Da):78,732
Last modified:January 4, 2005 - v1
Checksum:i521DE6ACFB25F40E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA Translation: CAI06550.1
RefSeqiWP_011236283.1, NC_006513.1

Genome annotation databases

EnsemblBacteriaiCAI06550; CAI06550; ebA819
KEGGieba:ebA819

Similar proteinsi

Entry informationi

Entry nameiMASZ_AROAE
AccessioniPrimary (citable) accession number: Q5P811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 4, 2005
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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