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Protein

Malate synthase G

Gene

glcB

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi429 – 4291MagnesiumUniRule annotation
Binding sitei429 – 4291GlyoxylateUniRule annotation
Metal bindingi457 – 4571MagnesiumUniRule annotation
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei633 – 6331Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAARO76114:GJTA-434-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:AZOSEA04280
ORF Names:ebA819
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GPRO_1000056892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei619 – 6191Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA819.

Structurei

3D structure databases

ProteinModelPortaliQ5P811.
SMRiQ5P811. Positions 1-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni454 – 4574Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5P811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERIQCNQL QVDAALHRFI ENEALPGTGL EAAAFWKGFS ELANELAPKN
60 70 80 90 100
RALLAERDRL QAEIDTWHRA HPGPIQDMPA YRAFLESIGY LQPVPAPFKI
110 120 130 140 150
TTANVDSEIS EQAGPQLVVP IMNARYALNA ANARWGSLYD ALYGTNAIPS
160 170 180 190 200
DGGAEAGSSY NPVRGARVIA FARNLLDQAA PLAQGSHDKT KAYAVAGGKL
210 220 230 240 250
IVTLLDGSQT GLADPAKFAG FQGDAAAPSA VLLKNNGLHL EIQFDRKHPI
260 270 280 290 300
GKTDAAGIKD VLVEAAVTTI MDCEDSTAAV DAEDKVVVYR SWLGLITGTL
310 320 330 340 350
VETFDKGGKT VERKMNPDRE YTAPNGSALR LHGRSLLFIR HVGHLMTNPA
360 370 380 390 400
ILLADGAEIP EGIMDGVVTT LISMRDLKLK RNSRTDSMYI VKPKMHGPAE
410 420 430 440 450
VAFASELFGR VEQLLGLPAD TMKVGIMDEE RRTSVNLAGC IKAAESRVAF
460 470 480 490 500
INTGFLDRTG DEMHTAMEAG PMIRKGDMKG SAWIQAYERQ NVLVGLDCGL
510 520 530 540 550
RGRAQIGKGM WAMPDLMAEM LEQKIGHPKA GATTAWVPSP TGATLHALHY
560 570 580 590 600
HQVDVQSVQQ EMEKISLAAE RDTLLDNLLT VPVAASANWS ATEIQQELDN
610 620 630 640 650
NAQGILGYVV RWIDQGVGCS KVPDINNIGL MEDRATLRIS SQHIANWLRH
660 670 680 690 700
GVTNEAQVLE TMKRMAAVVD QQNAGDPLYR PMAPDFDQSV AFQAAKDLVF
710 720
KGCAQPSGYT EPLLHRWRQV AKARG
Length:725
Mass (Da):78,732
Last modified:January 4, 2005 - v1
Checksum:i521DE6ACFB25F40E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI06550.1.
RefSeqiYP_157451.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI06550; CAI06550; ebA819.
KEGGieba:ebA819.
PATRICi20966895. VBIAroAro98752_0421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI06550.1.
RefSeqiYP_157451.1. NC_006513.1.

3D structure databases

ProteinModelPortaliQ5P811.
SMRiQ5P811. Positions 1-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76114.ebA819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI06550; CAI06550; ebA819.
KEGGieba:ebA819.
PATRICi20966895. VBIAroAro98752_0421.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciAARO76114:GJTA-434-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiMASZ_AROAE
AccessioniPrimary (citable) accession number: Q5P811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 4, 2005
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.