Reviewed, UniProtKB/Swiss-Prot Q5P801 (ACEK_AZOSE)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	AZOSEA04380
ORF Names:	ebA835

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288282
Regions
	Nucleotide binding	322 – 328	7	ATP By similarity
Sites
	Active site	378	1	By similarity
	Binding site	343	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P801-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4714B8FCE749C2B7	FASTA	591	69,162
	10 20 30 40 50 60 MDASVGENPV AQSIAQAMIE GFNKHYRIFR ETSRRAKESF EAAEWQAQID AVRERVQFYD 70 80 90 100 110 120 ERVDEAVRRL HEEFDADSLD DSTWQQLKLQ YIGILMRHKQ PELAETFFNS VCCKILHRTY 130 140 150 160 170 180 FNNDYLFARP AVSTEYIESY PPVYSSYYPQ DEGLRTTVRR IIEDFDWQRP FANLDRDIDN 190 200 210 220 230 240 ILRAVHEHIG AWPDMEVNCQ IQVLYSAFYR NKTAYIIGKA INGYQEYPFA LAVRHNPAGR 250 260 270 280 290 300 LEADTILLDP WRISVLFSLS RAYFLVDMEV PSGYVHFLRS IMPNKHRSEL YTMLGLGKQG 310 320 330 340 350 360 KTMFFRDLIA HLRHSNDQFI IAPGIRGLVM LVFTLPSYPY VFKIIKDVFG ASKNMDRATV 370 380 390 400 410 420 KRKYLMVKQV DRVGRLADTL EFSYAALPLS RFHPELLDEL RALAPSSFEI EGDSVIIKHL 430 440 450 460 470 480 YIERRMTPLN IYLEHADDDQ VEYAVREYGN AIRELATANI FPGDMLWKNF GVTRYGRVVF 490 500 510 520 530 540 YDYDEIEFMT AMNFRRIPPA PYPEMEMAAE PWYSAGPMDV FPEEFATFLL GAPRVRKAFL 550 560 570 580 590 KHHRDLLDAK FWQDVQASIR KGYLEDFFPY PTELRFCNMW SNERGRAHQA A

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06560.1.
RefSeq	YP_157461.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P801.
Genome annotation databases
GeneID	3182369.
GenomeReviews	Gene locus AZOSEA04380 in contig CR555306_GR.
KEGG	eba:ebA835.
NMPDR	fig|76114.4.peg.1187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P801.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	ASP76114:EBA835-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_AZOSE

Accession

Primary (citable) accession number: Q5P801

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)