Reviewed, UniProtKB/Swiss-Prot Q5P7X9 (SYFA_AZOSE)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	AZOSEA04600
ORF Names:	ebA868

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000231961
Sites
	Metal binding	264	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P7X9-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 94C0172B2F45F53F	FASTA	343	38,199
	10 20 30 40 50 60 MDKLDQLVQQ AAAEFAAAAD GVQLEQAKAR YLGKTGALTE RLKGLGKLAA EERRVAGAEI 70 80 90 100 110 120 NRAKDAIEAA LEARRQALRE AVLVAQLAAE ALDVTLPGRG AAQGGLHPVS RTLERIEALF 130 140 150 160 170 180 RSIGFVVADG PEVETDWHNF TALNTPENHP ARSMHDTFYL EGHEDVLLRT HTSPVQIRTM 190 200 210 220 230 240 LDHVARYGDR ESMPEIRVIV PGRVYRVDSD ATHSPMFHQV EGLWVGESVS FADLKGVIAD 250 260 270 280 290 300 FLRKFFETEE LQVRFRPSFF PFTEPSAEID VAFMSGALKG RWLEIAGCGM VHPNVLRFGG 310 320 330 340 IDPERYTGFA FGMGPDRLTM LRYGVNDLRL FFEGDLRFLS QFR

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06582.1.
RefSeq	YP_157483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P7X9.
Genome annotation databases
GeneID	3179432.
GenomeReviews	Gene locus AZOSEA04600 in contig CR555306_GR.
KEGG	eba:ebA868.
NMPDR	fig|76114.4.peg.1293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P7X9.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	ASP76114:EBA868-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AZOSE

Accession

Primary (citable) accession number: Q5P7X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)