Reviewed, UniProtKB/Swiss-Prot Q5P7X5 (SYT_AZOSE)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	AZOSEA04640
ORF Names:	ebA871

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_0000100934
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P7X5-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 1FA590EFCAB07ACA	FASTA	638	72,346
	10 20 30 40 50 60 MPNITLPDGS VRSFDHPVTV SEVASSIGAG LAKAALAGKV DGRLVDLSYR IEADTPLAIV 70 80 90 100 110 120 TEKGDEGLDV IRHSTAHLLA HAVKELFPEA QVTIGPVIEN GFYYDFAYKR PFTPEDLEKI 130 140 150 160 170 180 EKRMAELARR EIPVSREVWP RDKAVEFFKS QGEHYKAEII ASIPQAEDVS LYRQGDFIDL 190 200 210 220 230 240 CRGPHVPSTG KLKVFKLTKV AGAYWRGDSK NEMLQRIYGT AWAKKDDLEN YLHMLEEAEK 250 260 270 280 290 300 RDHRKLGRLL DLFHIQEEAP GMVFWHAKGW TLWQQVEQYM RRTILDNGYQ EVKTPQIVDR 310 320 330 340 350 360 SLWEKSGHWD MYSELMFTTQ SEKRDYAVKP MNCPCHIQIF NQGLKSYRDL PLRMAEFGSC 370 380 390 400 410 420 HRNEPSGALH GIMRVRNFVQ DDAHIFCADE QVQTEAAAFI ELLQKVYADF GFTEILIKLS 430 440 450 460 470 480 TRPDKRVGTD DQWDAAEAAL AAALDAQGLA YDLQPGEGAF YGPKIEFSLK DCLNRVWQCG 490 500 510 520 530 540 TLQLDFNLPV RLGAEYVAED NAKHYPVMLH RAILGSLERF IGILIEHYAG ALPLWLAPVH 550 560 570 580 590 600 AVVLNISEGQ TDYATEVARR LKQAGFRVEA DLRNEKINYK IREHSVHKLP YQIVIGEKEK 610 620 630 AAGVVAVRAR GGQDLGQMPL DTLIERWQRE IEARSGSI

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06586.1.
RefSeq	YP_157487.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P7X5.
Genome annotation databases
GeneID	3181320.
GenomeReviews	Gene locus AZOSEA04640 in contig CR555306_GR.
KEGG	eba:ebA871.
NMPDR	fig|76114.4.peg.1297.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P7X5.
OMA	QDEAPGM.
Enzyme and pathway databases
BioCyc	ASP76114:EBA871-MON.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AZOSE

Accession

Primary (citable) accession number: Q5P7X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)