Reviewed, UniProtKB/Swiss-Prot Q5P7U2 (SYY_AZOSE)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	AZOSEA04970
ORF Names:	ebA931

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Tyrosyl-tRNA synthetase HAMAP MF_02007		PRO_0000236691
Regions
	Domain	337 – 398	62	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02007
	Motif	226 – 230	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P7U2-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: C90C93C4F1F77761	FASTA	399	44,458
	10 20 30 40 50 60 MTDVQAALEL IKRGAEELLV EAELVEKLES DRPLRVKAGF DPTAPDLHLG HTVLLNKLRH 70 80 90 100 110 120 FQELGHQVMF LVGDFTAMIG DPSGKNATRP PLSREQILEN ARTYQEQVFK ILDPDKTEIC 130 140 150 160 170 180 FNSSWMESLG TAGMIRLASR YTVARMLERD DFAKRYAGGQ AIAIHEFLYP LCQGYDSVAM 190 200 210 220 230 240 RADVELGGTD QKFNLLVGRE LQKHDRQAPQ CVLMMPLLEG LDGVNKMSKS LGNYIGITEA 250 260 270 280 290 300 PREIFGKIMS ISDSLMWRYF DLLSFHPAVE IARYRAEVEG GRNPRELKVL LAQEIVARFH 310 320 330 340 350 360 SHAAAEDALA DFEARFQRGV LPDDIPEVNV VVGDGGLPVF QVVKQAGLTG STSEALRMIE 370 380 390 QGAVRLNGER VEDKGLVLQR DQTVVLQVGK RKFASVVLN

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI06619.1.
RefSeq	YP_157520.1.
3D structure databases
SMR	Q5P7U2. Positions 5-399.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P7U2.
Genome annotation databases
GeneID	3179684.
GenomeReviews	Gene locus AZOSEA04970 in contig CR555306_GR.
KEGG	eba:ebA931.
NMPDR	fig|76114.4.peg.1430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	YVVQVGK.
Enzyme and pathway databases
BioCyc	ASP76114:EBA931-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_AZOSE

Accession

Primary (citable) accession number: Q5P7U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)