Reviewed, UniProtKB/Swiss-Prot Q5P7T1 (SYP_AZOSE)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AZOSEA05080
ORF Names:	ebA950

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248641

Sequences

	Sequence		Length	Mass (Da)
	Q5P7T1-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 09D5032A103728A9	FASTA	583	64,296
	10 20 30 40 50 60 MRASQYFIST LKEAPSDAEV VSQKLMLRAG FIRKVAAGIY SYLPIGLRVI RKVEDIVRDE 70 80 90 100 110 120 MNRAGALELT MPLVQPAELW DETGRWEQMG AEMLRFKDRH QRDFALQPTS EEVVTDIARQ 130 140 150 160 170 180 ELKSYRQLPK NFYQIQTKFR DERRPRFGVM RGREFTMKDA YSFDRDVAAA GRSYDAMYAA 190 200 210 220 230 240 YCRIFDRLGL TYRAVAADTG AIGGDRSHEF QVIADTGEDA IVYCPGSDYA ANIELAEALP 250 260 270 280 290 300 LLATRAAPAQ ALEKTPTPGR TTCEEVAKLL GVPLETTVKS LVLATDDVDD TNKPAGVTVW 310 320 330 340 350 360 LLLVRGDHEL NEVKAGKIPG LKAGFRFATE TEIAERFGCQ PGYLGPVGVN KAVKIVADRT 370 380 390 400 410 420 VANMADFICG ANEADFHLTG TNWGRDLPEP DLVADLRNII EGDPSPDGRG RLAIQRGIEV 430 440 450 460 470 480 GHVFYLGTKY SQSMNATFLD ENGKPKHFEM GCYGIGVTRI LGAAIEQNHD ARGIIWPDAI 490 500 510 520 530 540 APFRVVVCPV GWGKSDAVRT EATKLYETLC AGGIDVILDD RDERPGVMFA DWELIGVPHR 550 560 570 580 VVIGDRGLKD GMAEYQGRRD AEAAKIPLAE LAAFVGSKLR PTA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06630.1.
RefSeq	YP_157531.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3181861.
GenomeReviews	Gene locus AZOSEA05080 in contig CR555306_GR.
KEGG	eba:ebA950.
NMPDR	fig|76114.4.peg.1441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P7T1.
OMA	Q5P7T1. VVSHQLM.
Enzyme and pathway databases
BioCyc	ASP76114:EBA950-MON.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AZOSE

Accession

Primary (citable) accession number: Q5P7T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)