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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:AZOSEA05180
ORF Names:ebA977
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
Proteomesi
  • UP000006552 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092271 – 938Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST938

Interactioni

Protein-protein interaction databases

STRINGi76114.ebA977.

Structurei

3D structure databases

ProteinModelPortaliQ5P7S1.
SMRiQ5P7S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 457Adenylyl removaseUniRule annotationAdd BLAST457
Regioni460 – 938Adenylyl transferaseUniRule annotationAdd BLAST479

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5P7S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEADAARLK TDRLGYNPLH VLRADAATRR QEPAILTMIR SSPPMSVDSS
60 70 80 90 100
PLPEALRHAA SISRFMRRML DSRPWLAAQL AGSLGQPLDS EAMSSFLANR
110 120 130 140 150
GVDEARLRAS LRHLRSWVVC HVLTRDLNGF ADLAEVTETM TVLAEVTVRA
160 170 180 190 200
TYEVLREGLV ARYGAPLSPT GWEQELLVIG MGKLGGRELN VSSDIDLIFV
210 220 230 240 250
YPEDGDTGGN KVISNFEFFE RLGKQMIQAL ADVTEHGQVF RVDMRLRPNG
260 270 280 290 300
DSGPLVCSFD MLENYFITQG REWERYAWIK ARVLAGERYH ELEQIARPFV
310 320 330 340 350
FRKYLDFGSI NAMRALHAQI RREVTRRDRV NNIKLGPGGI REIEFTAQVF
360 370 380 390 400
QLIRGGREPG LQVRATLDVL AVLGERGILT PQTVGELAEA YDFLRRLEHR
410 420 430 440 450
LQYLDDAQTH DLPGNDEDCE CIARAMDFPD YTSFLGVLDH HRAAVSRHFD
460 470 480 490 500
HVFGDPSEEA HTLDSMWAAA RDVEQAEPIL SRLGYGDPSG AAHRLAALHG
510 520 530 540 550
SARYQQLPNH IRSRLDALMP RVVEVAAATP GPDETLARCI DLIEAISRRG
560 570 580 590 600
AYLALLQQYP QALRRVADLV GASRWAAQYL TRHPILLDEL LDDRNLEPAP
610 620 630 640 650
DWDALRAQMR DTLDTLEPDM ERQMDVMREQ HHAQVFRLLT QDIAGLLTVE
660 670 680 690 700
KLADHLSALA DLMLELALPL VWRKIKIRHR DDPAFAVIAY GKLGGKELGY
710 720 730 740 750
ASDLDIVFLF DDPAPEALEV YTRLAQRINT WLSSQTAAGI LFETDLRLRP
760 770 780 790 800
NGESGLLVTS IESFRKYQLE SAWVWEHQAL TRARFAAGDA TIGAAFERIR
810 820 830 840 850
CEVLRLPRDI AALRAEVLAM RRKMSDAHAG KSERFNLKHD AGGLVDVEFL
860 870 880 890 900
IQYLVLGHAH RYPELTGNLG NIALLRIAGE LGLIPAELAA RCGDSYRLFR
910 920 930
RLQHRQRLNG LLSLVAPDEV AAAREPVRAL WTIVFGSA
Length:938
Mass (Da):105,278
Last modified:January 4, 2005 - v1
Checksum:iC26993BA14378E57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI06640.1.

Genome annotation databases

EnsemblBacteriaiCAI06640; CAI06640; ebA977.
KEGGieba:ebA977.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNE_AROAE
AccessioniPrimary (citable) accession number: Q5P7S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 4, 2005
Last modified: June 7, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families