Reviewed, UniProtKB/Swiss-Prot Q5P7Q3 (SYA_AZOSE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AZOSEA05360
ORF Names:	ebA1021

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075049

Sequences

	Sequence		Length	Mass (Da)
	Q5P7Q3-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 86A87402C56EFE3D	FASTA	873	95,217
	10 20 30 40 50 60 MKSAEIREKF LKFFESKGHQ IVASSSLVPH DDPTLLFTNA GMNQFKDVFL GFDKRPYSRA 70 80 90 100 110 120 TTSQKCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKHDAITYAW ELLTEEFGLP 130 140 150 160 170 180 KDKLWVTVYA DDDEAYDIWT KEIGVPAERV IRIGDNKGAR YASDNFWMMG DTGPCGPCTE 190 200 210 220 230 240 IFYDHGPEIW GGPPGSPDED GDRYIEIWNN VFMQFNRDEA GVMHPLPRPS VDTGMGLERV 250 260 270 280 290 300 SAVLQHVHSN YEIDLFQTLI RAAARETGCA DLDSPSLKVI ADHIRACSFL IADGVIPGNE 310 320 330 340 350 360 GRGYVLRRII RRAIRHGYKL GSRAAFFHRL VPDLVTEMGP AYPELKAGQA RVADVLKQEE 370 380 390 400 410 420 ERFFATIENG MAILETELAA MATAGSRTFN GETAFKLHDT YGFPLDLTAD VCRERDVTVD 430 440 450 460 470 480 AAAFDAAMAR QKEQARAAGK FRMAMNLEYE GPETKFHGYE RLEERGTVLA LYKDGAPVVE 490 500 510 520 530 540 LQEGDIGVVV LDNTPFYAES GGQVGDRGEL RGSAGIFEVE DTQKIQAAVY GHHGVVKTGR 550 560 570 580 590 600 LAVGQELGAR VDGEARASTQ RNHSVTHLMH KALREVLGAH VQQKGSQVDP DKTRFDFAHT 610 620 630 640 650 660 APLSPEEIRE VEDLVNAEIL ANVATEARVM SIDDAQKSGA MMLFGEKYGD EVRVLAIGSS 670 680 690 700 710 720 KELCGGTHVA RTGDIGLFKI VSEGGVAAGV RRIEAVTGAN ALRYAQEQER RVQGISALLK 730 740 750 760 770 780 VQPDEVAERI AGILDNVRAL EKELARMKSR LAASQGDELV AQAVEVRGAK VLAAMLEGAD 790 800 810 820 830 840 VATLRETLDK LKDKLKSAVI VLAAVADGRV SLIAGVTSDL TAKLKAGELV NMVAQQVGGK 850 860 870 GGGRPDMAQA GGTDPAHLPA ALASVKAWAE ARL

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI06658.1.
RefSeq	YP_157559.1.
3D structure databases
SMR	Q5P7Q3. Positions 1-462, 487-696.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P7Q3.
Genome annotation databases
GeneID	3181133.
GenomeReviews	Gene locus AZOSEA05360 in contig CR555306_GR.
KEGG	eba:ebA1021.
NMPDR	fig|76114.4.peg.1557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	TLMFTNS.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1021-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AZOSE

Accession

Primary (citable) accession number: Q5P7Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)