Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5P7M6

- BIOB_AROAE

UniProt

Q5P7M6 - BIOB_AROAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene

bioB

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi141 – 1411Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi273 – 2731Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAARO76114:GJTA-576-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:AZOSEA05630
ORF Names:ebA1066
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Biotin synthasePRO_0000381213Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA1066.

Structurei

3D structure databases

ProteinModelPortaliQ5P7M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiHESQIRI.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5P7M6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSAPAAERP AAPAVPRIKW TVVEAVELFE QPFMDLLFRA QQVHRAHFDP
60 70 80 90 100
NAVQRSTLLS IKTGGCSEDC GYCSQSTRHN TGLDREQLLK VAEVVEHAKA
110 120 130 140 150
AKAKGASRFC MGAAWRGPKD KDMETVLAMV REVKALGLET CVTLGMLKGD
160 170 180 190 200
QALQLKEAGL DYYNHNLDTA PEFYGQVITT HTLADRLDTL EQVRGAGINV
210 220 230 240 250
CSGGIVGMGE GRRSRAGLLV QLANMASPPE SVPINNLVPV PGTPLENVDK
260 270 280 290 300
IDPFEFVRTI AAARIMMPTS FVRLSAGRQQ MSDELQALCF MAGANSIFYG
310 320
DRLLTTDNPD TDRDEALFAR LGLRPV
Length:326
Mass (Da):35,517
Last modified:July 28, 2009 - v2
Checksum:i003736D8FE702CFA
GO

Sequence cautioni

The sequence CAI06685.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI06685.1. Different initiation.
RefSeqiYP_157586.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI06685; CAI06685; ebA1066.
GeneIDi3180751.
KEGGieba:ebA1066.
PATRICi20967199. VBIAroAro98752_0566.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI06685.1 . Different initiation.
RefSeqi YP_157586.1. NC_006513.1.

3D structure databases

ProteinModelPortali Q5P7M6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76114.ebA1066.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI06685 ; CAI06685 ; ebA1066 .
GeneIDi 3180751.
KEGGi eba:ebA1066.
PATRICi 20967199. VBIAroAro98752_0566.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi HESQIRI.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AARO76114:GJTA-576-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiBIOB_AROAE
AccessioniPrimary (citable) accession number: Q5P7M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: October 1, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3