Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5P7J0

- PDXA_AROAE

UniProt

Q5P7J0 - PDXA_AROAE

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei130 – 1301SubstrateUniRule annotation
    Binding sitei131 – 1311SubstrateUniRule annotation
    Metal bindingi160 – 1601Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi205 – 2051Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi260 – 2601Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei268 – 2681SubstrateUniRule annotation
    Binding sitei277 – 2771SubstrateUniRule annotation
    Binding sitei286 – 2861SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciAARO76114:GJTA-612-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Ordered Locus Names:AZOSEA05990
    ORF Names:ebA1139
    OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
    Taxonomic identifieri76114 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
    ProteomesiUP000006552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3263264-hydroxythreonine-4-phosphate dehydrogenasePRO_1000051490Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi76114.ebA1139.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5P7J0.
    SMRiQ5P7J0. Positions 7-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221592.
    KOiK00097.
    OMAiFHECARK.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5P7J0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLPILAIT SGEPAGIGPE LCAQLSRRDW PLRPVVLGDF ELIRARAAGA    50
    AVVRAYMPDA PAAAGVLDVL HVPLNVAARP GRLDPANAGY VLALLDRAVH 100
    GCRSGEFGAI VTAPVHKGVI NDAGVAFSGH TEYLAEHTGT PRVVMMLVGG 150
    GLRVALATTH LPLAAVPGAI TPALLDETLR ILHADLAGRF GLAAPRILVA 200
    GLNPHAGEGG HMGREEIDVI APVLERLRGE GMRLVGPLPA DTLFVPHTLG 250
    QGDAVLAMYH DQGLPVLKHA SFGGGVNVTL GLPIIRTSVD HGTALDLAGT 300
    GRADPGSLFA AVELATAMVC NRDVAR 326
    Length:326
    Mass (Da):33,572
    Last modified:January 4, 2005 - v1
    Checksum:i41823413D100D43A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI06721.1.
    RefSeqiYP_157622.1. NC_006513.1.

    Genome annotation databases

    EnsemblBacteriaiCAI06721; CAI06721; ebA1139.
    GeneIDi3181388.
    KEGGieba:ebA1139.
    PATRICi20967277. VBIAroAro98752_0605.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI06721.1 .
    RefSeqi YP_157622.1. NC_006513.1.

    3D structure databases

    ProteinModelPortali Q5P7J0.
    SMRi Q5P7J0. Positions 7-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 76114.ebA1139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI06721 ; CAI06721 ; ebA1139 .
    GeneIDi 3181388.
    KEGGi eba:ebA1139.
    PATRICi 20967277. VBIAroAro98752_0605.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221592.
    KOi K00097.
    OMAi FHECARK.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci AARO76114:GJTA-612-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
      Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
      Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EbN1.

    Entry informationi

    Entry nameiPDXA_AROAE
    AccessioniPrimary (citable) accession number: Q5P7J0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3