Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5P7J0 (PDXA_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxythreonine-4-phosphate dehydrogenase

EC=1.1.1.262
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene names
Name:pdxA
Ordered Locus Names:AZOSEA05990
ORF Names:ebA1139
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536

Catalytic activity

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536

Cofactor

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00536.

Miscellaneous

The active site is located at the dimer interface By similarity.

Sequence similarities

Belongs to the PdxA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3263264-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536
PRO_1000051490

Sites

Metal binding1601Divalent metal cation; shared with dimeric partner By similarity
Metal binding2051Divalent metal cation; shared with dimeric partner By similarity
Metal binding2601Divalent metal cation; shared with dimeric partner By similarity
Binding site1301Substrate By similarity
Binding site1311Substrate By similarity
Binding site2681Substrate By similarity
Binding site2771Substrate By similarity
Binding site2861Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5P7J0 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 41823413D100D43A

FASTA32633,572
        10         20         30         40         50         60 
MASLPILAIT SGEPAGIGPE LCAQLSRRDW PLRPVVLGDF ELIRARAAGA AVVRAYMPDA 

        70         80         90        100        110        120 
PAAAGVLDVL HVPLNVAARP GRLDPANAGY VLALLDRAVH GCRSGEFGAI VTAPVHKGVI 

       130        140        150        160        170        180 
NDAGVAFSGH TEYLAEHTGT PRVVMMLVGG GLRVALATTH LPLAAVPGAI TPALLDETLR 

       190        200        210        220        230        240 
ILHADLAGRF GLAAPRILVA GLNPHAGEGG HMGREEIDVI APVLERLRGE GMRLVGPLPA 

       250        260        270        280        290        300 
DTLFVPHTLG QGDAVLAMYH DQGLPVLKHA SFGGGVNVTL GLPIIRTSVD HGTALDLAGT 

       310        320 
GRADPGSLFA AVELATAMVC NRDVAR 

« Hide

References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR555306 Genomic DNA. Translation: CAI06721.1.
RefSeqYP_157622.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ5P7J0.
SMRQ5P7J0. Positions 7-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING76114.ebA1139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI06721; CAI06721; ebA1139.
GeneID3181388.
KEGGeba:ebA1139.
PATRIC20967277. VBIAroAro98752_0605.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1995.
HOGENOMHOG000221592.
KOK00097.
OMAFHECARK.
OrthoDBEOG6GN6ZC.

Enzyme and pathway databases

BioCycAARO76114:GJTA-612-MONOMER.
UniPathwayUPA00244; UER00312.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_00536. PdxA.
InterProIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00557. pdxA. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDXA_AROAE
AccessionPrimary (citable) accession number: Q5P7J0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways