Reviewed, UniProtKB/Swiss-Prot Q5P7C4 (PURA_AZOSE)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	AZOSEA06640
ORF Names:	ebA1249

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000224249
Regions
	Nucleotide binding	13 – 19	7	GTP Potential
Sites
	Active site	142	1	By similarity
	Active site	149	1	By similarity
	Metal binding	14	1	Magnesium By similarity
	Metal binding	41	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P7C4-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 64CAFD6245C296FD	FASTA	436	47,067
	10 20 30 40 50 60 MAKNVVVVGT QWGDEGKGKI VDWLTDHARG VVRFQGGHNA GHTLVIGANE YKLNLVPSGI 70 80 90 100 110 120 VREGVACYIG NGVVLDAHHL LSEIRTLEAG GIRVRDRLRI SPGCPLILGY HAALDRAREA 130 140 150 160 170 180 AKSAGDKIGT TGKGIGPTYE DKVARRALRV YDLFDRERFA AKLRANLDYH NFVLTQHLGA 190 200 210 220 230 240 EAVEFGSVFE QAMADAEEIR PMVADVSAEL YAVNKSGGSL LFEGAQGTLL DIDHGTYPFV 250 260 270 280 290 300 TSSNCVAGQA AAGSGVGPGR LHYVLGITKA YCTRVGGGPF PTELDIETAD TPGQQMSSKG 310 320 330 340 350 360 REFGTVTGRK RRCGWLDLGA LKRSIIINGV TGLCITKLDV LDGLSELKLC TGYMLDGRRI 370 380 390 400 410 420 DLLPMGSEEV TRCEPIFETM AGWSGTTFGA QSWEALPQEA RAYLHRIEEI CEIAIDVIST 430 GPERDETILR RHPFGA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06787.1.
RefSeq	YP_157688.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3181837.
GenomeReviews	Gene locus AZOSEA06640 in contig CR555306_GR.
KEGG	eba:ebA1249.
NMPDR	fig|76114.4.peg.1977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P7C4.
OMA	Q5P7C4. YVLGIIK.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1249-MON.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_AZOSE

Accession

Primary (citable) accession number: Q5P7C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)