Reviewed, UniProtKB/Swiss-Prot Q5P794 (HIS4_AZOSE)

Names and origin

Protein names

Recommended name:
    1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
    EC=5.3.1.16
Alternative name(s):
    Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	AZOSEA06940
ORF Names:	ebA1293

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the hisA/hisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP MF_01014		PRO_0000229041
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	131	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P794-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 899CB253115FE6A2	FASTA	249	26,145
	10 20 30 40 50 60 MLLIPAIDLK DGHCVRLKQG EMDDATVFSE DPAAMARHWI EQGARRLHLV DLNGAFAGKP 70 80 90 100 110 120 KNGAAIRSIV DEVGDDIPVQ LGGGVRDLDT IEHYLDNGIS YIIIGTAAVK NPGFLHDACG 130 140 150 160 170 180 AFPGHIIVGL DARDGKVAVD GWSKMTGHDV VDLAKKYEDY GVEAVIYTDI GRDGMLSGVN 190 200 210 220 230 240 VEATVRLAQA LRIPVIASGG IASIADIDAL CAVEAEGIMG AITGRAIYEG ALDFAVAQAR ADELNGGEG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06817.1.
RefSeq	YP_157718.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3179641.
GenomeReviews	Gene locus AZOSEA06940 in contig CR555306_GR.
KEGG	eba:ebA1293.
NMPDR	fig|76114.4.peg.2007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P794.
OMA	Q5P794. VATRGWL.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1293-MON.
Family and domain databases
HAMAP	MF_01014. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006063. His4. IPR006062. His_biosynth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00007. His4. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_AZOSE

Accession

Primary (citable) accession number: Q5P794

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)