Reviewed, UniProtKB/Swiss-Prot Q5P725 (ISPE_AZOSE)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	AZOSEA07630
ORF Names:	ebA1405

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235061
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	17	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P725-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: ED224D5921A4C019	FASTA	284	30,581
	10 20 30 40 50 60 MDTAELTRLA DCPAPAKLNL FLHVIGRRPD GYHLLQTAFR LLDWGDALSF ELRHDGVVAR 70 80 90 100 110 120 ATDLPGVSAE HDLVVRAARL LQSYTGCRLG ADIRVDKRLP MGGGLGGGSS DAATTLIALN 130 140 150 160 170 180 RLWRTGVRGE ELTALGLRLG ADVPFFIFGR DAFAEGVGDE LRPLDLPPAW YVVVAPPVTV 190 200 210 220 230 240 PTVEIFAAGE LTRDTEPIKI TDFAASTTRN DLQAVACSRY PEIGAAIDWL AQFAPARMTG 250 260 270 280 SGACVFAQVA SEDDAERIVA SCPGSYRAWK ARSVMQHPLR GWVG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06886.1.
RefSeq	YP_157787.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P725.
Genome annotation databases
GeneID	3182875.
GenomeReviews	Gene locus AZOSEA07630 in contig CR555306_GR.
KEGG	eba:ebA1405.
NMPDR	fig|76114.4.peg.2254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P725.
OMA	PMGGGIG.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1405-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_AZOSE

Accession

Primary (citable) accession number: Q5P725

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)