Reviewed, UniProtKB/Swiss-Prot Q5P6Z8 (DDL_AZOSE)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	AZOSEA07900
ORF Names:	ebA1442

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	D-alanine--D-alanine ligase		PRO_0000341056
Regions
	Domain	109 – 304	196	ATP-grasp
	Nucleotide binding	135 – 190	56	ATP By similarity
Sites
	Metal binding	258	1	Magnesium or manganese 1 By similarity
	Metal binding	271	1	Magnesium or manganese 1 By similarity
	Metal binding	271	1	Magnesium or manganese 2 By similarity
	Metal binding	273	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P6Z8-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 19BE2E8E1B3EB6A5	FASTA	309	32,762
	10 20 30 40 50 60 MRGNGVGAKF GKVAVLLGGT SAEREVSLMS GGAVLAALQA AGVDAHGFDP AQQDLHILKE 70 80 90 100 110 120 QGFDRAFIAL HGRGGEDGTV QGLLELLKIP YTGSGVMASA LAMDKWRTKM VWAACGLPTP 130 140 150 160 170 180 RYAILAGDTD WEAVVAELGL PIFVKPVHEG SSMGATKVTA ASQLKAAWER AARFDDLVLA 190 200 210 220 230 240 EEFIVGAELT VPFLAERALP VIRIEAPGGK YDYQNKYFTD ETRYLCPSGL PAEQEQALQA 250 260 270 280 290 300 LVMKSARALG CRGWGRADLM LTGDGRPYLL EMNTSPGMTG HSLVPMAAKA VGLDFTALCL AILEDARLG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06913.1.
RefSeq	YP_157814.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3180137.
GenomeReviews	Gene locus AZOSEA07900 in contig CR555306_GR.
KEGG	eba:ebA1442.
NMPDR	fig|76114.4.peg.2392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P6Z8.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1442-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_AZOSE

Accession

Primary (citable) accession number: Q5P6Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 4, 2005
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)