Reviewed, UniProtKB/Swiss-Prot Q5P6Z7 (MURC_AZOSE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	AZOSEA07910
ORF Names:	ebA1443

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000242542
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5P6Z7-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 74938679B5C05DD9	FASTA	471	49,707
	10 20 30 40 50 60 MKHKVKRIHF VGIGGAGMSG IAEVLANLGF MVSGSDLGEN AATRRLAALG VQVRVGHAAE 70 80 90 100 110 120 NVAGADAVVI STAVRNDNPE VVAARSRHVP IVPRAQMLAE LMRLKHGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVASILAEG GMDPTFVIGG KLNAAGANAR LGKGDFLVAE ADESDASFLL LTPVISVITN 190 200 210 220 230 240 IDADHMDTYG HDFARLKQAF VDFLQHLPFY GVAVLCEDDP HVRSIMPLVS KQVVRYGLSE 250 260 270 280 290 300 SANIRAENVR AEGGRMLFDV VRTNGSVSRL PIELNLPGVH NVLNALAAIA VATEVQVPDA 310 320 330 340 350 360 AIIKALAEFR GVGRRFQRYG EVALRDAEGT PQGSCTLIDD YGHHPVEMAA TLAAARGAFP 370 380 390 400 410 420 GRRLVLAFQP HRYTRTRDCF EDFVKVLSTV DALLLAEVYA AGEAPVVAAD GRSLARAIRV 430 440 450 460 470 AGKVEPVFVE DIDEMPATVL AAVRDGDVVI TMGAGSIGAV PGKLASSEEL V

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI06914.1.
RefSeq	YP_157815.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P6Z7.
Genome annotation databases
GeneID	3179325.
GenomeReviews	Gene locus AZOSEA07910 in contig CR555306_GR.
KEGG	eba:ebA1443.
NMPDR	fig|76114.4.peg.2393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P6Z7.
OMA	EWMVVEA.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1443-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AZOSE

Accession

Primary (citable) accession number: Q5P6Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)