Reviewed, UniProtKB/Swiss-Prot Q5P6Z4 (MURD_AZOSE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	AZOSEA07940
ORF Names:	ebA1447

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108958
Regions
	Nucleotide binding	122 – 128	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5P6Z4-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 2877A8A56F66AD8D	FASTA	466	48,375
	10 20 30 40 50 60 MSALTGKHVL VLGLGESGLA MARWCALRGA RLRVADSRQT PPGLDTLRAD APLAEIVVGN 70 80 90 100 110 120 FGAEVLSGID LVALSPGVDP RIGIAAEARH RGLPLTGEMS LLAQALDELG VRAQTRILAI 130 140 150 160 170 180 TGTNGKTTTT ALTAALAQAA GLDAVAAGNI SPAALDVLMD RLAQGAPLPE CWVLELSSFQ 190 200 210 220 230 240 IETMHALDPE AATVLNVTDD HLDRYAGLDE YAATKAAIFE GRGVQVLNRD DARVAAMALP 250 260 270 280 290 300 GRSLIRFGAS APAATDDYGL VEVAGAAWLV RGRERLLALA DLPLAGRHNA ANTLAALALC 310 320 330 340 350 360 EGGLGIAPQR LTGAVVAFRG LPHRVELVAE RSDGVRFYDD SKGTNVGATV AALDGMDCPV 370 380 390 400 410 420 VLIAGGDGKG QDFSPLREAV VRRARAVVLI GRDAPRIESA LQGSAVALEH APDLDAAVVR 430 440 450 460 ASEQAQPGDA VLLSPACASL DMFRNYAHRA EVFIAAARRL PEVRAS

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI06917.1.
RefSeq	YP_157818.1.
3D structure databases
SMR	Q5P6Z4. Positions 2-460.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P6Z4.
Genome annotation databases
GeneID	3182700.
GenomeReviews	Gene locus AZOSEA07940 in contig CR555306_GR.
KEGG	eba:ebA1447.
NMPDR	fig|76114.4.peg.2396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	DSKATNF.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1447-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_AZOSE

Accession

Primary (citable) accession number: Q5P6Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)