ID   PYRC_AZOSE              Reviewed;         344 AA.
AC   Q5P6Y5;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=Dihydroorotase;
DE            Short=DHOase;
DE            EC=3.5.2.3;
GN   Name=pyrC; OrderedLocusNames=AZOSEA08010; ORFNames=ebA1456;
OS   Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Aromatoleum.
OX   NCBI_TaxID=76114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA   Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA   Reinhardt R.;
RT   "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT   bacterium, strain EbN1.";
RL   Arch. Microbiol. 183:27-36(2005).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 3/6.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 1 subfamily.
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DR   EMBL; CR555306; CAI06926.1; -; Genomic_DNA.
DR   RefSeq; YP_157827.1; -.
DR   GeneID; 3181227; -.
DR   GenomeReviews; CR555306_GR; AZOSEA08010.
DR   KEGG; eba:ebA1456; -.
DR   NMPDR; fig|76114.4.peg.353; -.
DR   HOGENOM; Q5P6Y5; -.
DR   OMA; Q5P6Y5; IMPNLVP.
DR   BioCyc; ASP76114:EBA1456-MON; -.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR   GO; GO:0019856; P:pyrimidine base biosynthetic process; IEA:InterPro.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00219; -; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004721; DHOdimr.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   PIRSF; PIRSF001237; DHOdimr; 1.
DR   TIGRFAMs; TIGR00856; pyrC_dimer; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    344       Dihydroorotase.
FT                                /FTId=PRO_0000325570.
FT   METAL        13     13       Zinc 1 (By similarity).
FT   METAL        15     15       Zinc 1 (By similarity).
FT   METAL        99     99       Zinc 1; via carbamate group (By
FT                                similarity).
FT   METAL        99     99       Zinc 2; via carbamate group (By
FT                                similarity).
FT   METAL       136    136       Zinc 2 (By similarity).
FT   METAL       174    174       Zinc 2 (By similarity).
FT   METAL       247    247       Zinc 1 (By similarity).
FT   MOD_RES      99     99       N6-carboxylysine (By similarity).
SQ   SEQUENCE   344 AA;  37310 MW;  75E74BD36B3B7FC3 CRC64;
     MQTISLIRPD DWHLHVRDGA ALATVVPHTA RRFGRALIMP NLRPPVTTAA QALEYRARIL
     AAVPPGQHFE PLMSLYLTDN TSPDEIDRAK ASGAVVAVKL YPAGATTNSD AGVTAIEKVH
     AVLERMEKLG MVLCVHGEVT HGDVDVFDRE QVFIEQVLAP LVARFPALRV VFEHITTAEA
     ARFVQDAGPN VAATVTAHHL LLNRNAIFAG GIRPHHYCLP VLKRETHRRA LVEAATSGNA
     KFFLGTDSAP HSRAAKETAC GCAGCYTAHA AIELYAEAFE QAGALDRLEA FASLNGPAFY
     GLAPNAERIT LAKDAWEVPG EYEYLHDDPL VPLRAGETVA WRVL
//