Reviewed, UniProtKB/Swiss-Prot Q5P6F1 (ILVD2_AZOSE)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	AZOSEA09850
ORF Names:	ebA1822

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000225365
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5P6F1-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 091A992BE3DE991D	FASTA	616	65,701
	10 20 30 40 50 60 MPQYRSRTST AGRNMAGARA LWRATGMKDG DFEKPIIAIA NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHAGM LYSLPSRELI ADSVEYMVNA HTADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPA IFVSGGPMEA GKVKWEAKVI SLDLVDAMVK AADSSCSDAE 190 200 210 220 230 240 VDAIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGT VLATHADREQ LFLRAGRTIV 250 260 270 280 290 300 DMARRYYEKD DASVLPRSIV TFQAFENAIT LDVAMGGSTN TVLHLLAAAR EAGVDFTMAD 310 320 330 340 350 360 IDRISRKVPC LCKVAPAVAD VHIEDVHRAG GIMAILGELN RAGLLHAELP TVHSKTMAEA 370 380 390 400 410 420 LSRWDVMQAH DNAVFEFYKA APGGVPTQVA FSQNRRWKEL DMDRSHGVIR DRANAFTQEG 430 440 450 460 470 480 GLAVLYGNIA EKGCIVKTAG VDESIWTFTG RVRVFESQEA AVEGILGDRI VAGDIVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSYLK SKGLGKECAL LTDGRFSGGT SGLSIGHVSP EAACGGAIGL 550 560 570 580 590 600 VEEGDSIEID IPNRRIHLAV PDEVLTARRA AMNAKGHKAW KPEGRQRVVS AALQAYAALT 610 TSADTGGVRD VTQVQR

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI07110.1.
RefSeq	YP_158011.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P6F1.
Genome annotation databases
GeneID	3182001.
GenomeReviews	Gene locus AZOSEA09850 in contig CR555306_GR.
KEGG	eba:ebA1822.
NMPDR	fig|76114.4.peg.502.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P6F1.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	ASP76114:EBA1822-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_AZOSE

Accession

Primary (citable) accession number: Q5P6F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)