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Protein

(S)-1-Phenylethanol dehydrogenase

Gene

ped

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent stereospecific oxidation of (S)-1-phenylethanol to acetophenone in the degradation of ethylbenzene.2 Publications

Catalytic activityi

(S)-1-phenylethanol + NAD+ = acetophenone + NADH.2 Publications

Kineticsi

  1. KM=1.6 µM for (S)-1-phenylethanol1 Publication
  2. KM=51 µM for NAD+1 Publication
  3. KM=1 µM for acetophenone1 Publication
  4. KM=3.3 µM for NADH+1 Publication
  1. Vmax=1.4 µmol/min/mg enzyme with (S)-1-phenylethanol as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NAD1 Publication1
Binding sitei89NAD; via carbonyl oxygen1 Publication1
Binding sitei93NAD1 Publication1
Binding sitei141SubstrateBy similarity1
Active sitei154Proton acceptor1
Binding sitei158NAD1 Publication1
Binding sitei191NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 19NAD1 Publication3
Nucleotide bindingi61 – 63NAD1 Publication3
Nucleotide bindingi184 – 187NAD1 Publication4

GO - Molecular functioni

GO - Biological processi

  • anaerobic ethylbenzene catabolic process Source: UniProtKB
  • oxidation-reduction process Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1364.
BRENDAi1.1.1.311. 604.

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-1-Phenylethanol dehydrogenase (EC:1.1.1.311)
Gene namesi
Name:ped
Ordered Locus Names:AZOSEA13030
ORF Names:c1A58
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
Proteomesi
  • UP000006552 Componenti: Chromosome

Pathology & Biotechi

Biotechnological usei

The reverse reaction is of biotechnological interest for the specific production of chiral alcohols from ketones.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004187492 – 249(S)-1-Phenylethanol dehydrogenaseAdd BLAST248

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi76114.c1A58.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 7Combined sources4
Beta strandi9 – 13Combined sources5
Turni14 – 16Combined sources3
Helixi18 – 29Combined sources12
Beta strandi33 – 40Combined sources8
Helixi43 – 51Combined sources9
Beta strandi56 – 60Combined sources5
Helixi66 – 80Combined sources15
Beta strandi85 – 88Combined sources4
Helixi98 – 100Combined sources3
Helixi103 – 113Combined sources11
Helixi115 – 131Combined sources17
Beta strandi134 – 139Combined sources6
Helixi142 – 145Combined sources4
Helixi152 – 172Combined sources21
Helixi173 – 175Combined sources3
Beta strandi177 – 184Combined sources8
Helixi196 – 202Combined sources7
Beta strandi209 – 211Combined sources3
Helixi218 – 227Combined sources10
Helixi230 – 232Combined sources3
Beta strandi239 – 245Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EW8X-ray2.10A/B/C/D1-249[»]
2EWMX-ray2.40A/B1-249[»]
ProteinModelPortaliQ5P5I4.
SMRiQ5P5I4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5P5I4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108KEZ. Bacteria.
ENOG410XRY7. LUCA.
KOiK14746.
OrthoDBiPOG091H085B.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5P5I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRLKDKLA VITGGANGIG RAIAERFAVE GADIAIADLV PAPEAEAAIR
60 70 80 90 100
NLGRRVLTVK CDVSQPGDVE AFGKQVISTF GRCDILVNNA GIYPLIPFDE
110 120 130 140 150
LTFEQWKKTF EINVDSGFLM AKAFVPGMKR NGWGRIINLT STTYWLKIEA
160 170 180 190 200
YTHYISTKAA NIGFTRALAS DLGKDGITVN AIAPSLVRTA TTEASALSAM
210 220 230 240
FDVLPNMLQA IPRLQVPLDL TGAAAFLASD DASFITGQTL AVDGGMVRH
Length:249
Mass (Da):26,662
Last modified:January 4, 2005 - v1
Checksum:i2CA2967B5B6B32C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI07428.1.
RefSeqiWP_011237148.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI07428; CAI07428; c1A58.
KEGGieba:c1A58.
PATRICi20968729. VBIAroAro98752_1324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI07428.1.
RefSeqiWP_011237148.1. NC_006513.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EW8X-ray2.10A/B/C/D1-249[»]
2EWMX-ray2.40A/B1-249[»]
ProteinModelPortaliQ5P5I4.
SMRiQ5P5I4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76114.c1A58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI07428; CAI07428; c1A58.
KEGGieba:c1A58.
PATRICi20968729. VBIAroAro98752_1324.

Phylogenomic databases

eggNOGiENOG4108KEZ. Bacteria.
ENOG410XRY7. LUCA.
KOiK14746.
OrthoDBiPOG091H085B.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1364.
BRENDAi1.1.1.311. 604.

Miscellaneous databases

EvolutionaryTraceiQ5P5I4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPED_AROAE
AccessioniPrimary (citable) accession number: Q5P5I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.