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Q5P4B8

- LIPA_AROAE

UniProt

Q5P4B8 - LIPA_AROAE

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Protein
Lipoyl synthase
Gene
lipA, AZOSEA17200, ebA3050
Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi92 – 921Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi95 – 951Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAARO76114:GJTA-1743-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:AZOSEA17200
ORF Names:ebA3050
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Lipoyl synthaseUniRule annotation
PRO_0000325230Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76114.ebA3050.

Structurei

3D structure databases

ProteinModelPortaliQ5P4B8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiTIRAVRH.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5P4B8-1 [UniParc]FASTAAdd to Basket

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MDSTARKQRG ADKTARIPIK IVPAERLKKP EWIRIRLGAG HEAERFNEIK    50
ASLREHKLHT VCEEASCPNI HECFGKGTAT FMIMGDICTR RCPFCDVGHG 100
RPEPLNANEP QELAATIGAM RLNYVVITSV DRDDLRDGGA QHFVDCIRET 150
RAASPKTRIE VLVPDFRGRL DVALEIFDQA PPDVMNHNLE TVPRLYKQAR 200
PGADYAYSLR LLKEFKARHP DVPTKSGLMV GLGETDDEIL DVLRDLRAHD 250
VEMLTIGQYL QPSTGHLPVL RYVHPDTFKM FETEALAMGF KNAACGPMVR 300
SSYWADQQAY GAGVV 315
Length:315
Mass (Da):35,292
Last modified:January 4, 2005 - v1
Checksum:i524A8393B53C7E47
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI07845.1.
RefSeqiYP_158746.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI07845; CAI07845; ebA3050.
GeneIDi3182936.
KEGGieba:ebA3050.
PATRICi20969539. VBIAroAro98752_1726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR555306 Genomic DNA. Translation: CAI07845.1 .
RefSeqi YP_158746.1. NC_006513.1.

3D structure databases

ProteinModelPortali Q5P4B8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76114.ebA3050.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI07845 ; CAI07845 ; ebA3050 .
GeneIDi 3182936.
KEGGi eba:ebA3050.
PATRICi 20969539. VBIAroAro98752_1726.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi TIRAVRH.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AARO76114:GJTA-1743-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiLIPA_AROAE
AccessioniPrimary (citable) accession number: Q5P4B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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