Reviewed, UniProtKB/Swiss-Prot Q5P4A4 (DNAE2_AZOSE)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	AZOSEA17340
ORF Names:	ebA3077

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	1070 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1070	1070	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103365

Sequences

	Sequence		Length	Mass (Da)
	Q5P4A4-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 659B91336FE4881E	FASTA	1,070	117,515
	10 20 30 40 50 60 MDSAPPSPSP SPAPAYAELH CLTNFSFQRG ASHPEELVAR AAEAGYTALA ITDECSLAGV 70 80 90 100 110 120 VRAHQQLRRL ARPLKLLIGS ELRLVDGLRV VLLATDRAAY GRLARLITIG RRAATKGRYQ 130 140 150 160 170 180 LTCADLDRGI PGCLALVVPP DDRILDAAAL VADARWVGER FPDAAWLAVE LACGPDDGRR 190 200 210 220 230 240 LEALLAIASA AGLPPVAATG ALMHDPSRRA LADVLAALRL RVTIAEAGRA LAPNAERALH 250 260 270 280 290 300 ERAVLARRYP AELLAETLRI VARCHFRLDE LRYEYPAELV PDGETPASWL RRLVEDGLRW 310 320 330 340 350 360 RHGRPQDDLA PPKVRAQVEH ELALIAELGF EAYFLTVEDL VRFARGRGIL CQGRGSAANS 370 380 390 400 410 420 VICWALGITE VDPELGIMLV ERFISKERDE PPDIDVDFEH ERREEVIQYV YRKYGRERAA 430 440 450 460 470 480 LAATVIRYRA RSALRDVGRA LGLPPGQLER LARDRFWFDA GRIRPERLRE AGFDPASPGV 490 500 510 520 530 540 QRLATLTEAL VGFPRHLSQH VGGFVIARGR LDELVPVENA AMPERTVIQW DKDDLDALGL 550 560 570 580 590 600 LKIDVLALGM LSALRRSLAL VSTWRGRPFT LADIPREAPE VYRMLSEADS IGVFQIESRA 610 620 630 640 650 660 QMTMLPRLKP ARFYDLVVQV AIVRPGPIQG GMVHPYLQAR ARIERDEPVS YPQPHCPDAD 670 680 690 700 710 720 ADGTCVPRPD DVRRVLERTL GVPIFQEQVM QLAVVAAGFT PGEADQLRRA MGAFRRHGEL 730 740 750 760 770 780 ERYRAKLLAG MAARGYRAEF AERLCDQIEG FGSYGFPESH AASFALLVYC SAWLKCCEPA 790 800 810 820 830 840 AFLCGLLNSQ PMGFYAPSQL IQDARRHGVV VLGAEVTASD WDCTLEALPD ASPGAAPAVR 850 860 870 880 890 900 LGLQLVKGFG REAAMRIVAA RTERPFPNVD ELALRARLDA AALKRLAEAG ALEPLAGHRR 910 920 930 940 950 960 QALWQAAAGH APEGVLRGAR IAEPRAELAA PSEAQELVAD YARLGFTLGR HPLALLRAKL 970 980 990 1000 1010 1020 TTLRFVTAAE IRDYPDRKLA RIAGLVTCRQ RPGTAKGTLF LTVEDETGLA NVIVQAELVE 1030 1040 1050 1060 1070 RQRREVLGAR LLGVFGQIRS KGQVVHLLAQ RLVDHSALLG ALEARSRDFQ

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI07859.1.
RefSeq	YP_158760.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3179816.
GenomeReviews	Gene locus AZOSEA17340 in contig CR555306_GR.
KEGG	eba:ebA3077.
NMPDR	fig|76114.4.peg.957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P4A4.
OMA	Q5P4A4. MGFYSPD.
Enzyme and pathway databases
BioCyc	ASP76114:EBA3077-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_AZOSE

Accession

Primary (citable) accession number: Q5P4A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)