Reviewed, UniProtKB/Swiss-Prot Q5P4A1 (LEXA_AZOSE)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	AZOSEA17370
ORF Names:	ebA3086

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	LexA repressor HAMAP MF_00015		PRO_0000170001
Regions
	DNA binding	32 – 52	21	H-T-H motif By similarity
Sites
	Active site	121	1	For autocatalytic cleavage activity By similarity
	Active site	158	1	For autocatalytic cleavage activity By similarity
	Site	86 – 87	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P4A1-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 802F1EE3FF9BCE90	FASTA	203	22,207
	10 20 30 40 50 60 MISRADPLTA RQAEILDFIR HTVESEGRPP TRAEICTAFG FRSPNAAETH LRTLAAKGAI 70 80 90 100 110 120 VLEEGRARGI RLVEALGLPL VGHVAAGRPM LAVEHIEARY QIDSALFSPR ADYLLRVRGM 130 140 150 160 170 180 SMRDAGIIDS DLLAVHRTPQ VRAGQVVVAR LEDEVTVKTF TREGPIVRLL PANPDFEPIV 190 200 VDTRHQALDI EGIAVGLVRN GSR

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI07862.1.
RefSeq	YP_158763.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P4A1.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	3181911.
GenomeReviews	Gene locus AZOSEA17370 in contig CR555306_GR.
KEGG	eba:ebA3086.
NMPDR	fig|76114.4.peg.960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P4A1.
OMA	KVIGVFR.
Enzyme and pathway databases
BioCyc	ASP76114:EBA3086-MON.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_AZOSE

Accession

Primary (citable) accession number: Q5P4A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)