Q5P3R2 (MURB_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	AZOSEA19270
ORF Names:	ebA3410

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224653
Regions
	Domain	27 – 198	172	FAD-binding PCMH-type
Sites
	Active site	174	1	By similarity
	Active site	248	1	Proton donor By similarity
	Active site	344	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P3R2 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: A9CDE4AF0450B34E	FASTA	348	37,108
	10 20 30 40 50 60 MPGNSGAAPL PALEPHADLQ ALNTFGLPAR ADRLAIVEHP AQLAALLAQP DWAGAPRLVL 70 80 90 100 110 120 GGGSNLVLTG DFHGLVLKVA IGGRRLVGDD DDAWYVEAGA GENWHDFVRW TLAQGWPGLE 130 140 150 160 170 180 NLSLIPGTVG AAPIQNIGAY GLEVAERFAS LAVVSLETGA SFRLEAADCR FGYRDSIFKH 190 200 210 220 230 240 ELAGRTLVTS VTFRLPKRWL PVIRYADVAE ELAARGSVAP GPIDVSDAVI AIRRRKLPDP 250 260 270 280 290 300 AAIGNVGSFF KNPVIDAATL GRLVAGHPDL PHYLQPDGSV KLAAGWLIDR CGWKGRDLGP 310 320 330 340 VGVYERQALV LVNRGGATGT DVRRIAQAIR DDVSRRFGVD LEPEPVFV

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08052.1.
RefSeq	YP_158953.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5P3R2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P3R2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3180722.
GenomeReviews	Gene locus AZOSEA19270 in contig CR555306_GR.
KEGG	eba:ebA3410.
NMPDR	fig|76114.4.peg.1796.
PATRIC	20969992. VBIAroAro98752_1949.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	IEQAGWK.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	ASP76114:EBA3410-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_AROAE

Accession

Primary (citable) accession number: Q5P3R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program