Reviewed, UniProtKB/Swiss-Prot Q5P3K0 (ALR_AZOSE)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	AZOSEA19890
ORF Names:	ebA3534

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Alanine racemase HAMAP MF_01201		PRO_1000065970
Sites
	Active site	33	1	Proton acceptor; specific for D-alanine By similarity
	Active site	250	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	33	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P3K0-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: BB1BA9A18FE16FBA	FASTA	353	38,208
	10 20 30 40 50 60 MRPARALIDL DALRHNYHLA RSRHGGRALA VVKANAYGHG AAQCARALAA DADGFAVGFL 70 80 90 100 110 120 EEALELRAAG IDQPILLLEG VFAPAELDEV VRHDLWIVVH HAEQLRIIDH ARPALPLEVW 130 140 150 160 170 180 LKMNSGMNRA GFLSHELRPA WQRLKDSGKV GGITLMTHFA RADEPQVLAT TEQLTAFDTA 190 200 210 220 230 240 TRELPGPRSL ANSGAILGWP AAHRDWARPG ILLYGADPMP DEPNGLRPVM TLESAVIAVR 250 260 270 280 290 300 EIPAGAPLGY GARFHAERAT RAGLVALGYA DGYPRSVPNG TPVAVDGLRT RLIGRVSMDL 310 320 330 340 350 LTIDLTDLPD AGLGSRVELW GANIPVNRIA QAAKTISYEL LCNVKRVRFE YSG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI08114.1.
RefSeq	YP_159015.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P3K0.
Genome annotation databases
GeneID	3179926.
GenomeReviews	Gene locus AZOSEA19890 in contig CR555306_GR.
KEGG	eba:ebA3534.
NMPDR	fig|76114.4.peg.2069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P3K0.
OMA	ARECAVG.
Enzyme and pathway databases
BioCyc	ASP76114:EBA3534-MON.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_AZOSE

Accession

Primary (citable) accession number: Q5P3K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)