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Q5P3C5 (RUVC_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:AZOSEA20640
ORF Names:ebA3657
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence CAI08189.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000225119

Sites

Metal binding161Magnesium By similarity
Metal binding751Magnesium By similarity
Metal binding1471Magnesium By similarity
Metal binding1501Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5P3C5 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: F8A012F6400D0B99

FASTA18519,252
        10         20         30         40         50         60 
MKSASTVVAT RILGLDPGLR ITGFGVIDKL GNQLRYVASG CIRTRDGELP GRLKTLLDGV 

        70         80         90        100        110        120 
REVVAAYTPD QVAVEKVFVN VNPQSTLLLG QARGAVICGA VSCDLPVHEY TALQVKQAVV 

       130        140        150        160        170        180 
GYGKAAKEQV QHMVQRLLAL DGCPSPDAAD ALACAICHAH GGQGTAGAFA GRRRAGRILV 


APDPG 

« Hide

References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR555306 Genomic DNA. Translation: CAI08189.1. Different initiation.
RefSeqYP_159090.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ5P3C5.
SMRQ5P3C5. Positions 11-164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING76114.ebA3657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI08189; CAI08189; ebA3657.
GeneID3181627.
KEGGeba:ebA3657.
PATRIC20970268. VBIAroAro98752_2087.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAARGACIT.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycAARO76114:GJTA-2082-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_AROAE
AccessionPrimary (citable) accession number: Q5P3C5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 14, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families