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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateUniRule annotation1
Metal bindingi233ManganeseUniRule annotation1
Metal bindingi253Manganese; via tele nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Active sitei277UniRule annotation1
Metal bindingi302ManganeseUniRule annotation1
Binding sitei398GTPUniRule annotation1
Binding sitei429GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 281GTPUniRule annotation6
Nucleotide bindingi522 – 525GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAARO76114:G1GHY-2264-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:AZOSEA22910
ORF Names:ebA4040
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
Proteomesi
  • UP000006552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001035891 – 614Phosphoenolpyruvate carboxykinase [GTP]Add BLAST614

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA4040

Structurei

3D structure databases

ProteinModelPortaliQ5P2P8
SMRiQ5P2P8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 226Substrate bindingUniRule annotation3
Regioni396 – 398Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5P2P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHRFSEAAL AATPTYVRHE GLKKWVAGIA ALTEPERVVW CDGSEEEYDR
60 70 80 90 100
LCTEMVDAGM LIKLNPEKRK NSYLAWSDPS DVARVEDRTY ICSKDKADAG
110 120 130 140 150
PTNNWEDPAK MRDTLNGLFA GSMRGRTMYV IPFSMGPLGS PIAHIGIEIS
160 170 180 190 200
DSPYVVTNMR TMTRMGRAVF DVLGTDGEFV PCVHSVGAPL GDGEKDSRWP
210 220 230 240 250
CNPDTKYIVH FPETREIWSY GSGYGGNALL GKKCFALRIA STMARDEGWL
260 270 280 290 300
AEHMLILGVE SPEGEKTYVA AAFPSACGKT NFAMLIPPKS FNGWKITTIG
310 320 330 340 350
DDIAWIKPGK DGKFYAINPE AGFFGVAPGT SEKTNYNAMA TLKENIIFTN
360 370 380 390 400
VALTDDGDVW WEGMSKEAPA HLIDWQGNDW TPEIAKETGR KAAHPNSRFT
410 420 430 440 450
APASQCPSVD PQWEDPAGVP ISAFIFGGRR ATTVPLVYEA FNWNFGVYMA
460 470 480 490 500
ATLGSETTAA AFGAQGVVRR DPFAMLPFCG YHMGDYFNHW LRMGHVVEDT
510 520 530 540 550
PKIFCVNWFR MNERGEFMWP GFGENMRVLK WIVDRVRGRV GGKETGLGWM
560 570 580 590 600
PKFEDIDWTG ADVTREQFEA LTSVDADAWK KELALHKEWF DKLQDRLPRE
610
LVLKRELFEL TLAV
Length:614
Mass (Da):68,596
Last modified:January 4, 2005 - v1
Checksum:i11085013F685B7F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA Translation: CAI08416.1
RefSeqiWP_011238103.1, NC_006513.1

Genome annotation databases

EnsemblBacteriaiCAI08416; CAI08416; ebA4040
KEGGieba:ebA4040

Similar proteinsi

Entry informationi

Entry nameiPCKG_AROAE
AccessioniPrimary (citable) accession number: Q5P2P8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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