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Q5P2P8

- PCKG_AROAE

UniProt

Q5P2P8 - PCKG_AROAE

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei84 – 841SubstrateUniRule annotation
    Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
    Metal bindingi233 – 2331ManganeseUniRule annotation
    Binding sitei233 – 2331SubstrateUniRule annotation
    Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Active sitei277 – 2771UniRule annotation
    Metal bindingi302 – 3021ManganeseUniRule annotation
    Binding sitei398 – 3981GTPUniRule annotation
    Binding sitei429 – 4291GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2816GTPUniRule annotation
    Nucleotide bindingi522 – 5254GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAARO76114:GJTA-2311-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:AZOSEA22910
    ORF Names:ebA4040
    OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
    Taxonomic identifieri76114 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
    ProteomesiUP000006552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 614614Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103589Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi76114.ebA4040.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5P2P8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni396 – 3983Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5P2P8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNHRFSEAAL AATPTYVRHE GLKKWVAGIA ALTEPERVVW CDGSEEEYDR    50
    LCTEMVDAGM LIKLNPEKRK NSYLAWSDPS DVARVEDRTY ICSKDKADAG 100
    PTNNWEDPAK MRDTLNGLFA GSMRGRTMYV IPFSMGPLGS PIAHIGIEIS 150
    DSPYVVTNMR TMTRMGRAVF DVLGTDGEFV PCVHSVGAPL GDGEKDSRWP 200
    CNPDTKYIVH FPETREIWSY GSGYGGNALL GKKCFALRIA STMARDEGWL 250
    AEHMLILGVE SPEGEKTYVA AAFPSACGKT NFAMLIPPKS FNGWKITTIG 300
    DDIAWIKPGK DGKFYAINPE AGFFGVAPGT SEKTNYNAMA TLKENIIFTN 350
    VALTDDGDVW WEGMSKEAPA HLIDWQGNDW TPEIAKETGR KAAHPNSRFT 400
    APASQCPSVD PQWEDPAGVP ISAFIFGGRR ATTVPLVYEA FNWNFGVYMA 450
    ATLGSETTAA AFGAQGVVRR DPFAMLPFCG YHMGDYFNHW LRMGHVVEDT 500
    PKIFCVNWFR MNERGEFMWP GFGENMRVLK WIVDRVRGRV GGKETGLGWM 550
    PKFEDIDWTG ADVTREQFEA LTSVDADAWK KELALHKEWF DKLQDRLPRE 600
    LVLKRELFEL TLAV 614
    Length:614
    Mass (Da):68,596
    Last modified:January 4, 2005 - v1
    Checksum:i11085013F685B7F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI08416.1.
    RefSeqiYP_159317.1. NC_006513.1.

    Genome annotation databases

    EnsemblBacteriaiCAI08416; CAI08416; ebA4040.
    GeneIDi3179524.
    KEGGieba:ebA4040.
    PATRICi20970738. VBIAroAro98752_2318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI08416.1 .
    RefSeqi YP_159317.1. NC_006513.1.

    3D structure databases

    ProteinModelPortali Q5P2P8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 76114.ebA4040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI08416 ; CAI08416 ; ebA4040 .
    GeneIDi 3179524.
    KEGGi eba:ebA4040.
    PATRICi 20970738. VBIAroAro98752_2318.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci AARO76114:GJTA-2311-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
      Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
      Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EbN1.

    Entry informationi

    Entry nameiPCKG_AROAE
    AccessioniPrimary (citable) accession number: Q5P2P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3