Q5P286 (ARGB_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	AZOSEA24530
ORF Names:	ebA4330

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000264681
Regions
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Binding site	94	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Site	37	1	Transition state stabilizer By similarity
	Site	257	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P286 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E1AFC1FD89D64B73	FASTA	300	31,740
	10 20 30 40 50 60 MPQLPAPTDV TPARKAEILA EALPYIKRFF DKTVVIKYGG NAMTDPHLKD CFARDVVLLK 70 80 90 100 110 120 LVGLNPVVVH GGGPQIETLL ARVGKKGEFI QGMRVTDAET MEVVEMVLGG QVNKEIVSLI 130 140 150 160 170 180 NQHGGKAVGL TGKDASFIRA KKLLMQKEDA APGDVVDVGQ VGEITKIDPS LIAFLDQGDF 190 200 210 220 230 240 IPVIAPIGVG EAGETYNINA DVVAGKLAEI LKAEKLVLLT NTPGVLDKAG KLLTGLTPHQ 250 260 270 280 290 300 IDDLVADGTL SGGMLPKISS ALDAARNGVK SVHIIDGRVE HCLLLEILTD HGVGTMIKSK

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08578.1.
RefSeq	YP_159479.1. NC_006513.1.
3D structure databases
HSSP	HSSP built from PDB template 2BUF based on UniProtKB Q9HTN2.
ProteinModelPortal	Q5P286.
SMR	Q5P286. Positions 13-300.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3181048.
GenomeReviews	Gene locus AZOSEA24530 in contig CR555306_GR.
KEGG	eba:ebA4330.
NMPDR	fig|76114.4.peg.2751.
PATRIC	20971078. VBIAroAro98752_2483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	GGNAMID.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4330-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_AROAE

Accession

Primary (citable) accession number: Q5P286

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program