Q5P280 (SYD_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	AZOSEA24590
ORF Names:	ebA4339

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110819

Sequences

	Sequence		Length	Mass (Da)
	Q5P280 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 1D84067B9143FB3A	FASTA	599	67,170
	10 20 30 40 50 60 MRTHYCGQVT AADLDQTVTL CGWVHRRRDH GGVIFIDLRD REGLVQVVCD PDRAETFHTA 70 80 90 100 110 120 ESIRNEFVIE LTGKVRRRPA GTENPNLVSG EIEVLCHTLE VLNASATPPF QLDDDNLSEN 130 140 150 160 170 180 VRLTHRVIDL RRPQMQKNLM LRYKVAMAFR RFLDAQGFID VETPMLTKST PEGARDYLVP 190 200 210 220 230 240 SRVHPGQFFA LPQSPQLFKQ LLMVAGFDRY YQITKCFRDE DLRADRQPEF TQVDIETSFL 250 260 270 280 290 300 DEAEITAIME DMIRYVFREA LAVELPNPFP RMTHAEAMRR YGSDKPDLRV TLELTDVTDA 310 320 330 340 350 360 VQDVAFKVFS GPATSGGRVA ALRVPGGASL TRGEIDEYTK FVGIYGARGL AYIKVNDVTK 370 380 390 400 410 420 PNDEGLQSPI VKNLHEEALR TILERTGAES GDLIFFGADK TKVVNDALGA LRTKLGHEKG 430 440 450 460 470 480 YVSGAAWTPV WVVDFPMFEY DDESKRWVAC HHPFTAPKDE HVELLESAPG ECLAKAYDLA 490 500 510 520 530 540 LNGWEIGGGS VRIHRADMQS KVFRALNIGD EEAQLKFGFL LDALKYGAPP HGGLAFGLDR 550 560 570 580 590 VVTLMTGAES IRDVIAFPKT QRAQCLLTDA PGEVDDKQLR ELHIRLRQKI ETQVEVAKA

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08584.1.
RefSeq	YP_159485.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5P280.
SMR	Q5P280. Positions 1-587.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P280.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3180955.
GenomeReviews	Gene locus AZOSEA24590 in contig CR555306_GR.
KEGG	eba:ebA4339.
NMPDR	fig|76114.4.peg.2756.
PATRIC	20971090. VBIAroAro98752_2489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4339-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AROAE

Accession

Primary (citable) accession number: Q5P280

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program