Q5P255 (PROA_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	AZOSEA24840
ORF Names:	ebA4380

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189685

Sequences

	Sequence		Length	Mass (Da)
	Q5P255 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 823329058BA020C3	FASTA	425	45,075
	10 20 30 40 50 60 MDIHDYMQTL GRQARAASRV LATASTADKN AALVAMAAAI REQTGELLAA NARDLDEARA 70 80 90 100 110 120 AGLEAALIDR LTLTAKGIEA MAEGLEQVAG LPDPIGEITD VKRRPSGIQV GKMRVPLGVI 130 140 150 160 170 180 GIIYEARPNV TADAAALCLK SGNAAILRGG KEALHCNQAI AQCVRTGLAA AGLPETSVQV 190 200 210 220 230 240 VETTDRAAVG HLITMPEFVD VIVPRGGKGL IERISKEARV PVIKHLDGNC HVYIDRDADV 250 260 270 280 290 300 AKVVPIVENA KTQRYGTCNT AESLLVAREV APRLLPEIGR MLATKGVEMR GCEQSLAILR 310 320 330 340 350 360 AAGIAADKLR AASEQDWSEE YLAPVIAVKV VADLDEAIAH INTYSSGHTE AIVTENYTSA 370 380 390 400 410 420 MRFLREVDSS SVMVNASTRF ADGFEYGLGA EIGISTDKIH ARGPVGLDGL TSQKWIVFGN GEIRG

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08609.1.
RefSeq	YP_159510.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5P255.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3179697.
GenomeReviews	Gene locus AZOSEA24840 in contig CR555306_GR.
KEGG	eba:ebA4380.
NMPDR	fig|76114.4.peg.2782.
PATRIC	20971140. VBIAroAro98752_2513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4380-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_AROAE

Accession

Primary (citable) accession number: Q5P255

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program