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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAARO76114:GJTA-2513-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:AZOSEA24870
ORF Names:ebA4386
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Leucine--tRNA ligasePRO_1000091289Add
BLAST

Proteomic databases

PRIDEiQ5P252.

Interactioni

Protein-protein interaction databases

STRINGi76114.ebA4386.

Structurei

3D structure databases

ProteinModelPortaliQ5P252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi628 – 6325"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5P252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKYTPAAV EATAQQHWES TQAFKVAEDA GKPKYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VLARYHRMRG FNVLQPMGWD AFGMPAENAA IQNNVPPAKW
110 120 130 140 150
TYANIDYMKT QLKRLGFALD WSRELATCKP DYYRWEQWLF TRLYQKGLIY
160 170 180 190 200
KKLGTVNWDP VDETVLANEQ VIDGRGWRSG ALIEKREIPM YYMKITAYAD
210 220 230 240 250
ELLEALDTLT GWPEQVKLMQ KNWIGRSEGV EVHFPYEVST IGASGVLKVF
260 270 280 290 300
TTRADTLMGA TYVAVAAEHP LALQAAVNDP ELAAFIEECR HGGVAEADLA
310 320 330 340 350
TMEKKGMPTG LRVVHPLTGE HLPVWIANYV LMGYGEGAVM AVPAHDERDF
360 370 380 390 400
AFATKYRLPI RMVVRSTRDA YTDTVAPWQD AYAEQGRLVN SGKYDGLHFH
410 420 430 440 450
DAIEAIAAEL TAKGLGAKRT QYRLRDWGIS RQRYWGCPIP MIHCADCGDV
460 470 480 490 500
PVPDEQLPVV LPEDVAVTGR GSPLAKMPRF YECDCPKCGK PAKRETDTMD
510 520 530 540 550
TFVESSWYFL RYASADNGQA MVDERVNYWA PVDQYIGGIE HAILHLLYSR
560 570 580 590 600
FFTRAMRDEG LVNVSEPFTN LLTQGMVVAE TYYRDADGGK KQWINPADVE
610 620 630 640 650
VERDEKGRIV AAKLTADGAP VVIGGIEKMA KSKNNGVDPQ ALVDQYGADT
660 670 680 690 700
ARLFIIFAAP PDQQLEWSDS GVEGAYRFLR RVWSFGHAFV SEFRPQLPAD
710 720 730 740 750
RQLDAVQLPE ALAAVRREIH VCLRQANYDF GKHQFNTVVS AAMKILNALE
760 770 780 790 800
KAPRDVAAAH AQVAEEGLDI LLRLLAPITP HVAHALWQDC GFTGDVLHAS
810 820 830 840 850
WPEPSEDALR QDEIDLVLQV NGKLRGSLRV AAGASNSAIE ALALGSETAQ
860 870
KFMEGKPPRK VVVVPGRLVN IVV
Length:873
Mass (Da):97,432
Last modified:January 4, 2005 - v1
Checksum:i17317CB2086C0B1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI08612.1.
RefSeqiWP_011238298.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI08612; CAI08612; ebA4386.
KEGGieba:ebA4386.
PATRICi20971146. VBIAroAro98752_2516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI08612.1.
RefSeqiWP_011238298.1. NC_006513.1.

3D structure databases

ProteinModelPortaliQ5P252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76114.ebA4386.

Proteomic databases

PRIDEiQ5P252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI08612; CAI08612; ebA4386.
KEGGieba:ebA4386.
PATRICi20971146. VBIAroAro98752_2516.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAARO76114:GJTA-2513-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiSYL_AROAE
AccessioniPrimary (citable) accession number: Q5P252
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 4, 2005
Last modified: July 22, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.