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Q5P239 (SYM_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:AZOSEA25000
ORF Names:ebA4404
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Methionine--tRNA ligase HAMAP MF_00098
PRO_0000139097

Regions

Domain608 – 718111tRNA-binding
Motif12 – 2211"HIGH" region HAMAP MF_00098
Motif349 – 3535"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1591Zinc By similarity
Binding site3521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5P239 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: B8147EB4F955FF36

FASTA71879,527
        10         20         30         40         50         60 
MPRKILVTNA LPYANGDIHL GHLVGYIQGD IWVRYQRMRG NTVHYVCADD THGTPVMLRA 

        70         80         90        100        110        120 
EKEGITPEAL IGRVHGEHLR DFTDFGVAFD NYHSTHSVEN RAYAEDVYTK LKAAELIDTR 

       130        140        150        160        170        180 
AIEQFYDPVK EMFLPDRFIK GECPKCGAAD QYGDNCEACG AAYAPTELKN PHSAVSGAKP 

       190        200        210        220        230        240 
VLKTSEHYFF RLSDPRAVAF LREWTRGTNA AGTRRLQVEA ANKMKEWLGD ETVGDGGNTL 

       250        260        270        280        290        300 
SDWDISRDAP YFGFEIPGAP GKYFYVWLDA PIGYFASFRN LAEQRGDIAV DDFTDAVRAE 

       310        320        330        340        350        360 
TAGTEMVHFI GKDILYFHAL FWPAMLRFAG YRTPTQLCVN GFLTVDGAKM SKSRGTFITA 

       370        380        390        400        410        420 
RSYVSRGLNP EWLRYYFATK SNGTMEDVDL SLDDMVAKVN SDLVGKYVNI ASRCAGFIAK 

       430        440        450        460        470        480 
RFDGKLGASD PQATADFEAA FAAGTIAHAY EERDYGRALR EIMRLADLAN QYVNDHKPWE 

       490        500        510        520        530        540 
LAKQEGQEAH LHVVCSTALT HFRDLTLYLK PVLPALAQKV EAFLAIDPLV WPQTWQPLPA 

       550        560        570        580        590        600 
AHTINPYAHL MTRVERKQID ALLEANRESL APAAPAAKVA SSQQRHAEKQ QHEAQSAETA 

       610        620        630        640        650        660 
MPHISIDDFT KVDLRIARIV GAEHVEGADK LIRLRLDIGE SENGQAKLRQ VFAGIKSAYD 

       670        680        690        700        710 
PATLVGRLTV MVANLAPRKM KFGVSEGMVL AASSADDKSS GIYLLSPDTG AASGMRVK

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References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR555306 Genomic DNA. Translation: CAI08625.1.
RefSeqYP_159526.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ5P239.
SMRQ5P239. Positions 136-161.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5P239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3180299.
GenomeReviewsGene locus AZOSEA25000 in contig CR555306_GR.
KEGGeba:ebA4404.
NMPDRfig|76114.4.peg.2798.
PATRIC20971174. VBIAroAro98752_2530.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMAHEIAPFK.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycASP76114:EBA4404-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_AROAE
AccessionPrimary (citable) accession number: Q5P239
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 4, 2005
Last modified: January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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