Reviewed, UniProtKB/Swiss-Prot Q5P233 (TYSY_AZOSE)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	AZOSEA25060
ORF Names:	ebA4415

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140915
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P233-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: CA9BC0C1FAE0629E	FASTA	264	30,134
	10 20 30 40 50 60 MKQYLDLMRH VLDHGDRKSD RTGTGTLSIF GWQMRFNLDD GFPLLTTKKL HTRSIIHELL 70 80 90 100 110 120 WFLRGDTNIR YLNENGVSIW DDWADENGDL GPVYGKQWRR WETANGATID QIAQLIDGLK 130 140 150 160 170 180 HNPDSRRHLV SAWNPGEVAG MALPPCHALF QFYVAGDRLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTLMVAQ VCGLRAGDFV WTGGDCHLYL NHLDQARLQL SRQPRALPRM IVNPGVTDIF 250 260 GFRFEDFRLE GYDPHPHIKA EVAV

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI08631.1.
RefSeq	YP_159532.1.
3D structure databases
SMR	Q5P233. Positions 2-264.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P233.
Genome annotation databases
GeneID	3182790.
GenomeReviews	Gene locus AZOSEA25060 in contig CR555306_GR.
KEGG	eba:ebA4415.
NMPDR	fig|76114.4.peg.2804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P233.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4415-MON.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_AZOSE

Accession

Primary (citable) accession number: Q5P233

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)