Q5P228 (DXS_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	AZOSEA25110
ORF Names:	ebA4439

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256372

Sequences

	Sequence		Length	Mass (Da)
	Q5P228 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 6019F4F108C57BFF	FASTA	621	67,579
	10 20 30 40 50 60 MPPYPLLHRI DSPFDLRKLD RRELPALAAE LRAFLIESVS KTGGHLSSNL GTVELSIALH 70 80 90 100 110 120 YIFDTPEDRI VWDVGHQTYG HKILTGRREA MSRLRQFGGI SGFPRRSESS YDTFGTAHSS 130 140 150 160 170 180 TSISAALGMA VAARNRGEQR RSIAVIGDGA MSAGMAFEAL NNAGDMRDIN LLVILNDNEM 190 200 210 220 230 240 SISPPVGALT KILARMFSGR TYNAARRAGE KVLGVSPPML DFARKVEEHV KGLITPGTLF 250 260 270 280 290 300 EEFGFHYYGP IDGHDLDALI PTLQNLRKLN GPHFLHVITR KGQGYKLAEA DPVLYHGVST 310 320 330 340 350 360 FDHTAGIQTA KGAAKLTYTQ VFGDWLCDIA AQDEKVIGIT PAMREGSGMV RFAQEFPNRY 370 380 390 400 410 420 YDVGIAEQHA LTFAAGLACE GFKPVVAIYS TFLQRAYDQL IHDIALQNLP VMFAIDRAGL 430 440 450 460 470 480 VGADGATHHG AFDLSYLGCI PNLVVMAPAD ENECRQMLYT AYRHNGPAAV RYPRGGGMQV 490 500 510 520 530 540 GPETAMSALP IGKGEIRRSG HRIALLVFGS LLFNALQAAE QLDATVANMR FVKPLDVTLI 550 560 570 580 590 600 EELAASHDLL VTLEENIVIG GAGSEVARVL ESLSDRPQLL RLGLPDTFID HGDQSQLLES 610 620 VGLDAPGIVA AIRRRFSPDN N

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08636.1.
RefSeq	YP_159537.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5P228.
SMR	Q5P228. Positions 1-616.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3179981.
GenomeReviews	Gene locus AZOSEA25110 in contig CR555306_GR.
KEGG	eba:ebA4439.
NMPDR	fig|76114.4.peg.2808.
PATRIC	20971208. VBIAroAro98752_2542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	DPILYHG.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4439-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AROAE

Accession

Primary (citable) accession number: Q5P228

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program