Reviewed, UniProtKB/Swiss-Prot Q5P221 (SYI_AZOSE)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	AZOSEA25180
ORF Names:	ebA4448

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	939 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 939	939	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_0000098343
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP MF_02002
	Motif	615 – 619	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	902	1	Zinc By similarity
	Metal binding	905	1	Zinc By similarity
	Metal binding	922	1	Zinc By similarity
	Metal binding	925	1	Zinc By similarity
	Binding site	574	1	Aminoacyl-adenylate By similarity
	Binding site	618	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P221-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 42837213399F6856	FASTA	939	105,391
	10 20 30 40 50 60 MADYRKTLNM PDTPFPMRGD LAKREPGWVA DWQAKKLYQK VRKAAAGRPK FVLHDGPPYA 70 80 90 100 110 120 NGDIHIGHAV NKILKDIIVR SKTLAGFDAP YVPGWDCHGL PIEHQIEKQH GKHLPADRAR 130 140 150 160 170 180 ELCREYAAEQ IERQKKDFIR LGVLGDWDNP YRTMNFSNEA DEIRALGELF RKGFLFKGLK 190 200 210 220 230 240 PVNWCFDCGS ALAEAEVEYQ DKKSPAIDVG FPLVDNERDK LAHAFGLDTL PEQPVYIVIW 250 260 270 280 290 300 TTTPWTIPSN QALNVHPELI YELVETPKGL LILAAELRAS ALERYQLEGR VLAAARGVAL 310 320 330 340 350 360 DRINFRHPFY DRLSPVFLGD YVAADAGTGI VHSAPAYGLD DFVSCTRYGM RNDEILNPVQ 370 380 390 400 410 420 ADGTFAGSLP FFGGLSVWDA NPKIVDKLAE VGSLFASGTL THSYMHCWRH KTPVIYRATT 430 440 450 460 470 480 QWFVGMDRLP GREAVEPGSA TLRELALKAV DETQFYPAWG KARLHSMIAN RPDWCVSRQR 490 500 510 520 530 540 NWGVPIPLFL HKETGEPHPR SLELLEQVAQ RVEQHGIDAW FKLDAAELLG SDVAQYDKMR 550 560 570 580 590 600 DTLDVWFDSG TTHWTVLRGS HAADSQWPAA LYLEGSDQHR GWFHSSLLTG CAIDGRAPYD 610 620 630 640 650 660 ALLTHGFVVD GQGKKMSKSK GNVVAPQEVS DKLGAEILRL WVAATDYSGE LTISKEILDR 670 680 690 700 710 720 VVEVYRRLRN TLRFLLANTG DFDIARDGVP VEEWLDIDHY ALALTRRLQE QVTGDYARFE 730 740 750 760 770 780 FHKIVQALQN FAAEDLGAFY VDILKDRLYT TKADSRARRA AQTALWHITQ AITRMMAPIL 790 800 810 820 830 840 TFTAEEIWRV TGNDAEDSVM LHTWHELPEL GASDEILARW ELIRSARAEV QKVLESLRSD 850 860 870 880 890 900 GRIGASLQAE VRVRASGARF DALASVGADL HFVLITSRAE LVRVETEADE GVDAAPSSHQ 910 920 930 KCGRCWHFRE DIGVDADHPE LCGRCCTNLH GDGESRTHA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI08643.1.
RefSeq	YP_159544.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P221.
Genome annotation databases
GeneID	3182804.
GenomeReviews	Gene locus AZOSEA25180 in contig CR555306_GR.
KEGG	eba:ebA4448.
NMPDR	fig|76114.4.peg.2815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P221.
OMA	FPMRGNL.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4448-MON.
Family and domain databases
HAMAP	MF_02002. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_AZOSE

Accession

Primary (citable) accession number: Q5P221

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)