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Q5P221

- SYI_AROAE

UniProt

Q5P221 - SYI_AROAE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei574 – 5741Aminoacyl-adenylateUniRule annotation
    Binding sitei618 – 6181ATPUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAARO76114:GJTA-2549-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:AZOSEA25180
    ORF Names:ebA4448
    OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
    Taxonomic identifieri76114 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
    ProteomesiUP000006552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098343Add
    BLAST

    Proteomic databases

    PRIDEiQ5P221.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi76114.ebA4448.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5P221.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi615 – 6195"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5P221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADYRKTLNM PDTPFPMRGD LAKREPGWVA DWQAKKLYQK VRKAAAGRPK    50
    FVLHDGPPYA NGDIHIGHAV NKILKDIIVR SKTLAGFDAP YVPGWDCHGL 100
    PIEHQIEKQH GKHLPADRAR ELCREYAAEQ IERQKKDFIR LGVLGDWDNP 150
    YRTMNFSNEA DEIRALGELF RKGFLFKGLK PVNWCFDCGS ALAEAEVEYQ 200
    DKKSPAIDVG FPLVDNERDK LAHAFGLDTL PEQPVYIVIW TTTPWTIPSN 250
    QALNVHPELI YELVETPKGL LILAAELRAS ALERYQLEGR VLAAARGVAL 300
    DRINFRHPFY DRLSPVFLGD YVAADAGTGI VHSAPAYGLD DFVSCTRYGM 350
    RNDEILNPVQ ADGTFAGSLP FFGGLSVWDA NPKIVDKLAE VGSLFASGTL 400
    THSYMHCWRH KTPVIYRATT QWFVGMDRLP GREAVEPGSA TLRELALKAV 450
    DETQFYPAWG KARLHSMIAN RPDWCVSRQR NWGVPIPLFL HKETGEPHPR 500
    SLELLEQVAQ RVEQHGIDAW FKLDAAELLG SDVAQYDKMR DTLDVWFDSG 550
    TTHWTVLRGS HAADSQWPAA LYLEGSDQHR GWFHSSLLTG CAIDGRAPYD 600
    ALLTHGFVVD GQGKKMSKSK GNVVAPQEVS DKLGAEILRL WVAATDYSGE 650
    LTISKEILDR VVEVYRRLRN TLRFLLANTG DFDIARDGVP VEEWLDIDHY 700
    ALALTRRLQE QVTGDYARFE FHKIVQALQN FAAEDLGAFY VDILKDRLYT 750
    TKADSRARRA AQTALWHITQ AITRMMAPIL TFTAEEIWRV TGNDAEDSVM 800
    LHTWHELPEL GASDEILARW ELIRSARAEV QKVLESLRSD GRIGASLQAE 850
    VRVRASGARF DALASVGADL HFVLITSRAE LVRVETEADE GVDAAPSSHQ 900
    KCGRCWHFRE DIGVDADHPE LCGRCCTNLH GDGESRTHA 939
    Length:939
    Mass (Da):105,391
    Last modified:January 4, 2005 - v1
    Checksum:i42837213399F6856
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI08643.1.
    RefSeqiYP_159544.1. NC_006513.1.

    Genome annotation databases

    EnsemblBacteriaiCAI08643; CAI08643; ebA4448.
    GeneIDi3182804.
    KEGGieba:ebA4448.
    PATRICi20971222. VBIAroAro98752_2549.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR555306 Genomic DNA. Translation: CAI08643.1 .
    RefSeqi YP_159544.1. NC_006513.1.

    3D structure databases

    ProteinModelPortali Q5P221.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 76114.ebA4448.

    Proteomic databases

    PRIDEi Q5P221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI08643 ; CAI08643 ; ebA4448 .
    GeneIDi 3182804.
    KEGGi eba:ebA4448.
    PATRICi 20971222. VBIAroAro98752_2549.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci AARO76114:GJTA-2549-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
      Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
      Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EbN1.

    Entry informationi

    Entry nameiSYI_AROAE
    AccessioniPrimary (citable) accession number: Q5P221
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3