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Q5P221

- SYI_AROAE

UniProt

Q5P221 - SYI_AROAE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei574 – 5741Aminoacyl-adenylateUniRule annotation
Binding sitei618 – 6181ATPUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAARO76114:GJTA-2549-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:AZOSEA25180
ORF Names:ebA4448
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098343Add
BLAST

Proteomic databases

PRIDEiQ5P221.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA4448.

Structurei

3D structure databases

ProteinModelPortaliQ5P221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi615 – 6195"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5P221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADYRKTLNM PDTPFPMRGD LAKREPGWVA DWQAKKLYQK VRKAAAGRPK
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAV NKILKDIIVR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHQIEKQH GKHLPADRAR ELCREYAAEQ IERQKKDFIR LGVLGDWDNP
160 170 180 190 200
YRTMNFSNEA DEIRALGELF RKGFLFKGLK PVNWCFDCGS ALAEAEVEYQ
210 220 230 240 250
DKKSPAIDVG FPLVDNERDK LAHAFGLDTL PEQPVYIVIW TTTPWTIPSN
260 270 280 290 300
QALNVHPELI YELVETPKGL LILAAELRAS ALERYQLEGR VLAAARGVAL
310 320 330 340 350
DRINFRHPFY DRLSPVFLGD YVAADAGTGI VHSAPAYGLD DFVSCTRYGM
360 370 380 390 400
RNDEILNPVQ ADGTFAGSLP FFGGLSVWDA NPKIVDKLAE VGSLFASGTL
410 420 430 440 450
THSYMHCWRH KTPVIYRATT QWFVGMDRLP GREAVEPGSA TLRELALKAV
460 470 480 490 500
DETQFYPAWG KARLHSMIAN RPDWCVSRQR NWGVPIPLFL HKETGEPHPR
510 520 530 540 550
SLELLEQVAQ RVEQHGIDAW FKLDAAELLG SDVAQYDKMR DTLDVWFDSG
560 570 580 590 600
TTHWTVLRGS HAADSQWPAA LYLEGSDQHR GWFHSSLLTG CAIDGRAPYD
610 620 630 640 650
ALLTHGFVVD GQGKKMSKSK GNVVAPQEVS DKLGAEILRL WVAATDYSGE
660 670 680 690 700
LTISKEILDR VVEVYRRLRN TLRFLLANTG DFDIARDGVP VEEWLDIDHY
710 720 730 740 750
ALALTRRLQE QVTGDYARFE FHKIVQALQN FAAEDLGAFY VDILKDRLYT
760 770 780 790 800
TKADSRARRA AQTALWHITQ AITRMMAPIL TFTAEEIWRV TGNDAEDSVM
810 820 830 840 850
LHTWHELPEL GASDEILARW ELIRSARAEV QKVLESLRSD GRIGASLQAE
860 870 880 890 900
VRVRASGARF DALASVGADL HFVLITSRAE LVRVETEADE GVDAAPSSHQ
910 920 930
KCGRCWHFRE DIGVDADHPE LCGRCCTNLH GDGESRTHA
Length:939
Mass (Da):105,391
Last modified:January 4, 2005 - v1
Checksum:i42837213399F6856
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI08643.1.
RefSeqiYP_159544.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI08643; CAI08643; ebA4448.
GeneIDi3182804.
KEGGieba:ebA4448.
PATRICi20971222. VBIAroAro98752_2549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI08643.1 .
RefSeqi YP_159544.1. NC_006513.1.

3D structure databases

ProteinModelPortali Q5P221.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76114.ebA4448.

Proteomic databases

PRIDEi Q5P221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI08643 ; CAI08643 ; ebA4448 .
GeneIDi 3182804.
KEGGi eba:ebA4448.
PATRICi 20971222. VBIAroAro98752_2549.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AARO76114:GJTA-2549-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiSYI_AROAE
AccessioniPrimary (citable) accession number: Q5P221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 4, 2005
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3