Q5P1H8 (SYC_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	AZOSEA27110
ORF Names:	ebA4792

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159342
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	269 – 273	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	272	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P1H8 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4BEC8BF9DBEA7612	FASTA	461	51,978
	10 20 30 40 50 60 MLHIHNTLTR RKEAFVPIAP GKVRMYVCGM TVYDYCHLGH ARVMVVFDMV ARWLRASGFE 70 80 90 100 110 120 LTYVRNITDI DDKIIRRAGE KGESIRTLTD RFIAAMHEDA DALGVLRPDH EPRATEYVMQ 130 140 150 160 170 180 MQSLIGRLRD KGLAYVAGNR DVCYSVRKFD SYGRFSGKSL DELRAGERVE VAGDKQDPLD 190 200 210 220 230 240 FVLWKHARTD EPDEVKWASP WGAGRPGWHI ECSAMSSDLL GEQFDIHGGG QDLQFPHHEN 250 260 270 280 290 300 EIAQSEGAHG HTFVNYWMHN GFVRVDDEKM SKSLGNFFTI RDVLERFDPE VVRFFILRAH 310 320 330 340 350 360 YRSPLNYSDA HLEDARQALT RLYTALRNVQ PSDAQVDWDE PHAMRFRAAM DDDFNTAEAV 370 380 390 400 410 420 AVLFELANEA NRSGSAATAA QLKGLGGVLG LLAREPASFL QGRMAGEPDK GEGVAIESMI 430 440 450 460 ERRALAKKSK DYAEADRIRA QLLASGIVLE DTPHGTVWRR A

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI08836.1.
RefSeq	YP_159737.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5P1H8.
SMR	Q5P1H8. Positions 1-400.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P1H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3180031.
GenomeReviews	Gene locus AZOSEA27110 in contig CR555306_GR.
KEGG	eba:ebA4792.
NMPDR	fig|76114.4.peg.2608.
PATRIC	20971618. VBIAroAro98752_2739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4792-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_AROAE

Accession

Primary (citable) accession number: Q5P1H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program