Reviewed, UniProtKB/Swiss-Prot Q5P1A9 (PYRD_AZOSE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	AZOSEA27800
ORF Names:	ebA4888

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000148422
Sites
	Active site	172	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P1A9-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 9BEC000BA69A3B7E	FASTA	350	37,333
	10 20 30 40 50 60 MLYNIARPLL FSLDPETAHE LSLAALHFFG RVLPAATPTE SDPVDVMGLH FPNRIGLAAG 70 80 90 100 110 120 LDKNGEAIDG LARLGFGFLE IGTITPRPQP GNPRPRMFRL PEVRAIINRM GFNNHGVDAL 130 140 150 160 170 180 VANVRAAKYR GILGINIGKN FDTPIDRAAD DYLACLDKVY ALASYVTVNI SSPNTKNLRQ 190 200 210 220 230 240 LQGESELDDL LGRLKARQQQ LADRHGRYVP LTLKIAPDLE PAQVTNIADA LRRHRIDGVI 250 260 270 280 290 300 ATNTTIARDK VQGVRYAEQQ GGLSGAPVFE ASTAVVAQLS RALAGELPII AAGGILDGRG 310 320 330 340 350 ARAKLAAGAS LVQVYSGLIY RGPSLIAECV RATTDFADSG HAATSASFGA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI08905.1.
RefSeq	YP_159806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P1A9.
Genome annotation databases
GeneID	3180158.
GenomeReviews	Gene locus AZOSEA27800 in contig CR555306_GR.
KEGG	eba:ebA4888.
NMPDR	fig|76114.4.peg.2676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P1A9.
OMA	AALNRMG.
Enzyme and pathway databases
BioCyc	ASP76114:EBA4888-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AZOSE

Accession

Primary (citable) accession number: Q5P1A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)